高电压技术2017，Vol.43Issue(8)：2454-2463,10.DOI:10.13336/j.1003-6520.hve.20170731003
基于阻抗谱的脉冲电场诱导兔肝脏组织不可逆电穿孔程度评估新方法的实验研究
Experimental Study on a Novel Method for Evaluating the Irreversible Electroporation Degree on Rabbit Liver Tissue Induced by Pulsed Electric Field Based on Impedance Spectrum
摘要
Abstract
The effectiveness and safety of irreversible electroporation (IRE) in tumor therapy has been proved by clinical applications for many years.The high-frequency irreversible electroporation (H-FIRE) proposed recently has both the advantages of the conventional IRE and the benefit of inhibiting the muscle contraction during the treatment.However,the mechanism of tumor ablation is unknown yet.We tried to analyze the difference between IRE and H-FIRE in tissue ablation based on impedance spectrum.The variation of the impedance specmum of the New Zealand White Rabbit liver was studied when treated by both conventional IRE and H-FIRE,and then the circuit model was used to fit the impedance curve and extract the information from the change of the impedance spectrum.The results showed that the impedance spectrum of the tissue changed significantly when treated by pulses and the impedance mode variated significantly in the low-middle frequency while the impedance phase changed significantly in the middle-high frequency with the direction from negative values to zero.After fitting the impedance data to the circuit model,the information showed that the change of the extracellular resistance decreased significantly because the new pathway for current was in parallel with the extracellular resistance.The change of the extracellular resistance induced by conventional IRE was larger than that induced by H-FIRE when they had the same pulse dose,and among the H-FIRE,the change would go with the increase of pulse duration.The variations of the impedance and the elements in the circuit model can reflect the degree of IRE accurately and is expected to be an effective approach to detect the outcome of IRE in clinical.关键词
高频不可逆电穿孔/组织消融/阻抗谱/肿瘤治疗/等效电路/脉冲电场Key words
high-frequency irreversible electroporation/tissue ablation/impedance spectrum/tumor therapy/equivalent circuit/pulsed electric field引用本文复制引用
姚陈果,赵亚军,董守龙,Suyashree Bhonsle,吕彦鹏,刘红梅,马剑豪,Rafael Davalos..基于阻抗谱的脉冲电场诱导兔肝脏组织不可逆电穿孔程度评估新方法的实验研究[J].高电压技术,2017,43(8):2454-2463,10.基金项目
国家创新研究群体基金(51321063) （51321063）
重庆市杰出青年基金(cstc2014jcyjjq90001) （cstc2014jcyjjq90001）
中央高校基本科研业务费资助(106112017CDJQJ158835).Project supported by Fund for Innovative Research Groups of China(51321063),Scientific Foundation for Outstanding Young Scientist of Chongqing (cstc2014jcyjjq90001),Fundamental Research Funds for the Central Universities (106112017CDJQJ158835). （106112017CDJQJ158835）
