高电压技术2017，Vol.43Issue(8)：2486-2495,10.DOI:10.13336/j.1003-6520.hve.20170731007
65dB以下变压器噪声暴露对SD大鼠神经递质和海马区神经组织的影响实验研究
Experimental Research on Effect of Transformer Noise Exposure Below 65 dB on the Neurotransmitter and Nervoustissue in Hippocampus of SD Rats
摘要
Abstract
To study the possible effect of the transformer noise exposure below 65 dB,as A weighting sound pressure level,on the neurotransmitter and nervoustissue in hippocampus of SD rats,we experimentally investigated the transformer noise in a 1 000 kV UHV substation,as noise experiment source.The noise samples were replayed in the noiseproof box.96 SD rats were grouped randomly into noise exposed group S1 (32),S2 (32),and control group C (32).The noise exposed group S1 was exposed to the 60 dB and the exposed group S2was exposed to the 65 dB transformer noise for consecutively 35 days,the transformer noise was diurnal equivalent as A weighting sound pressure level(Ldn).The control group was housed to the same conditions without the transformer noise.The back noise of the experimental box is less than 35 dB.The neurotransmitter and nervoustissue of the hippocampus area in SD rats were determined after a 35-day noise exposure,as well as the expression levels of CaN and CaMKII subunit.Neurotransmitter was determinated by high-performance liquid chromatography.Results revealed no significant difference (P＞0.05) between exposed group and control group with Glu,GABA,5-HT and DA.Ultmstructure of neurons and synapses in hippocampus of SD rats were observed by transmission electron microscopy.No significant difference(P＞0.05) was found in the neuronal ultrastructure and synaptic ultrastructure.Western blot was used to detect the expression levels of CaN and CaMKII subunit.And there was no significant difference (P＞0.05) in protein expression assay (expression of CaN A and CaMKII α subunit).Exposure of the 60 dB and 65 dB transformer noise in 35 days does not affect the SD rat hippocampus neuronal apoptosis and expression of CaN and CaMKII.The transformer noise about the substation is less than 65 dB daytime as a weighting sound pressure level,and 55 dB at night.The main frequency component is below 500 Hz.High frequency components are low and decaying fast.This steady noise is much lower than traffic and factory noise which is not enough to cause changes in neurotransmitter and hippocampal tissue structure in SD rats.In conclusion,60 dB and 65 dB of transformer noise (Ldn) have no effect on the neurotransmitter and nervoustissue in hippocampus of SD rats.关键词
变压器噪声/A计权声压级/神经递质/神经组织/海马区/SD大鼠Key words
transformer noise/A weighting sound pressure level/neurotransmitter/nervoustissue/hippocampus/SD rat引用本文复制引用
刘兴发,唐雨萌,周兵,刘震寰,万保权,张建功,李炜,曲敏,唐利军..65dB以下变压器噪声暴露对SD大鼠神经递质和海马区神经组织的影响实验研究[J].高电压技术,2017,43(8):2486-2495,10.基金项目
国家电网公司科技项目(GY71-16-008) （GY71-16-008）
湖北省科技支撑计划(2014BEC065).Project supported by Science and Technology Project of SGCC (GY71-16-008),Hubei Provincial Science and Technology Supporting Program of China (2014BEC065). （2014BEC065）
