高电压技术2017，Vol.43Issue(8)：2513-2517,5.DOI:10.13336/j.1003-6520.hve.20170731010
纳秒脉冲消融乳腺癌及免疫激活效应的实验研究
Experimental Study on Immune Stimulation Effect Caused by Nanosecond Pulsed Electric Fields Ablation on Breast Cancer
摘要
Abstract
During recent decades of years,animal experiments and preclinical studies have shown that nanosecond pulsed electric fields (nsPEFs) have characteristics with high energy,minimally invasive,approach without combined chemotherapy drugs.Consequently,we applied nsPEFs to MDA-MB-231 breast cancer bearing mice to observe the ablation effect of local primary tumor.The immune stimulation effect of local ablation on the T cell subsets were explored to investigate its immune mechanism as a non-thermal ablation.The mice with normal immune function were subcutaneously inoculated with breast cancer MDA-MB-231 cells for 2 weeks till the tumor diameter was around 10 mm.The nsPEFs treatment parameters were selected to be pulse duration 300 ns,electric field strength 40 kV/cm and pulse number 100 pulse,3 treatments every other day.The tumor formation rate was 100％.The tumor size,morphology and volume were followed up to 1 month.The animals were euthanized when the tumor volume was 20 mm.The ablation rate of the local tumor was 83％,and the volume of the primary tumor in ablation group was significantly lower than that of the control group.The flow cytometry was used to detect the spleen lymphocyte subsets.After ablation,the number and proportion of CD4 + T cells and CD8 + T cells in the spleen of mice were increased,suggesting that the localized nanosecond pulse stimulated the proliferation of spleen T cells.The results reveal that the nsPEF ablation can not only inhibit the primary tumor,but also enhance the immune function of mice,making the tumor growth and metastasis invasiveness decreased,help to inhibit tumor formation and development.关键词
纳秒脉冲/非热消融/乳腺癌/免疫激活效应/微创治疗Key words
nanosecond pulsed electric fields/non-thermal ablation/breast cancer/immune stimulation effect/minimally invasive treatment引用本文复制引用
陈璐艳,徐小波,洪良杰,陈翊玲,陈新华,苗旭东..纳秒脉冲消融乳腺癌及免疫激活效应的实验研究[J].高电压技术,2017,43(8):2513-2517,5.基金项目
国家自然科学基金(81372425) （81372425）
浙江省自然科学基金(LY17H160018) （LY17H160018）
新疆重点实验室合作项目(2014KL002).Project supported by National Natural Science Foundation of China(81372425),Natural Science Foundation of Zhejiang Province (LY17H160018),Cooperation Program of Xinjiang Key Laboratory (2014KL002). （2014KL002）
