广东药科大学学报2017，Vol.33Issue(4)：466-470,5.DOI:10.16809/j.cnki.2096-3653.2017041801
砂仁叶油的化学成分分析及促进创面愈合作用研究
Chemical constituents from Amomum villosum leaf oil and its activity of promoting wound healing
摘要
Abstract
Objective To analyze the chemical constituents from Amomum villosum leaf oil and investigate its effect on rabbit skin wound healing. Methods The Amomum villosum leaf oil was extracted by steam distillation and analyzed by GC-MS. The relative percentages were calculated with peak area normalization method. The wounds on the back skin of rabbits were prepared, and the wound healing rate was measured and calculated on the 3rd day, the 7th day and the 13th day after operation. After wound healing the wound specimens were collected for HE staining. Results 45 compounds were isolated and 43 compounds were identified. Amomum villosum leaf oil could significantly shorten the rabbit wound healing time and promoted wound healing with equivalent effect with nitrofurazone. Tissue section HE dyeing observation showed that Amomum villosum leaf oil could promote wound healing, and the newborn skin was in good condition with less inflammatory cells and tissue lesions. Conclusion The main components of Amomum villosum leaf oil consisted of pseudo limonene ( 41.12％) , 3-carene ( 24.17％) , d-limonene ( 3.25％) and myrtenol ( 3.18％) . Amomum villosum leaf oil could promote skin wound healing.关键词
砂仁叶油/化学成分/创面愈合Key words
Amomum villosum leaf oil/chemical constituents/wound healing分类
医药卫生引用本文复制引用
黄凤婷,万绵洁,张丹雁..砂仁叶油的化学成分分析及促进创面愈合作用研究[J].广东药科大学学报,2017,33(4):466-470,5.基金项目
国家科技部"十二五"国家科技支撑计划( 2011BAI01B02) （ 2011BAI01B02）
