广东药科大学学报2017，Vol.33Issue(4)：547-550,4.DOI:10.16809/j.cnki.2096-3653.2017050901
鞘氨醇激酶2(SphK2)基因敲除小鼠繁殖及基因型鉴定
Breeding and genotyping of Shingosine Kinase 2 knockout mice
摘要
Abstract
Objective To breed and identify SphK2 gene knock-out mice. Methods SphK2 knockout heterozygous mice were bred and DNA of the offspring mice was extracted. The genotypes of the offspring mice were analyzed by PCR. Results The feeding and breeding of SphK2 heterozygous mice were successful, and the offspring mice were obtained. The genotypes included heterozygous ( SphK2+/-) , homozygous ( SphK2-/-) and wild type ( SphK2+/+) . Conclusion The methods of breeding and genotyping of SphK2 knockout mice have been successful established, which provides an ideal animal model for further study of the biological function of SphK2.关键词
SphK2/基因敲除小鼠/基因型Key words
SphK2/gene knockout mice/genotype分类
医药卫生引用本文复制引用
李柠,李昌正,杨桂智,颜思珊,李锐,刘冰,兰天..鞘氨醇激酶2(SphK2)基因敲除小鼠繁殖及基因型鉴定[J].广东药科大学学报,2017,33(4):547-550,4.基金项目
广东省自然科学基金重点项目( 2016A030311014) （ 2016A030311014）
广东省科技计划项目( 2017A020211007,2014A020212460) （ 2017A020211007,2014A020212460）
