广东药科大学学报2017，Vol.33Issue(4)：551-555,5.DOI:10.16809/j.cnki.2096-3653.2017040501
TP X2在甲状腺乳头状癌中的表达及其对甲状腺癌KAT细胞增殖能力的影响
Investigation of the expression of TPX2 in thyroid carcinoma and its effect on KAT cell proliferation
关善斌 1黄新若 2李加伟 1黄天斌1
作者信息
- 1. 广西梧州市红十字会医院 普通外科二区,广西 梧州 543002
- 2. 广西梧州职业学院, 广西梧州543002
- 折叠
摘要
Abstract
Objective To investigate the expression of TPX2 in papillary thyroid carcinoma ( PTC) tissues and its correlation with clinical pathological parameters, as well as its effect on KAT cell proliferation. Methods 60 cases of PTC tissues and paired normal tissues were collected in our hospital during 2013-2016. The mRNA level of TPX2 was determined by qRT-PCR. KAT cells were transfected with special siRNA to inhibit TPX2 expression, and then cell proliferation, cell apoptosis and apoptosis-associated protein ( cleaved caspase 3, caspase 3 ) were tested by MTT, flow cytometry and Western blot. Results Compared to the paired normal tissues, TPX2 was highly expressed in tumor tissues at mRNA level ( 39/60) ( P<0.05) . Knockdown of TPX2 effectively reduced KAT cell growth and increased cell apoptosis. Meantime, apoptosis-related proteins cleaved caspase 3 and caspase 3 were up-regulated in TPX2-siRNA-treated group. Conclusion The expression of TPX2 is higher in PTC tissues. TPX2 is highly correlated with cell proliferation, which may be a potential target for treatment of thyroid carcinoma.关键词
甲状腺癌/TPX2/增殖/细胞凋亡Key words
thyroid carcinoma/TPX2/proliferation/cell apoptosis分类
医药卫生引用本文复制引用
关善斌,黄新若,李加伟,黄天斌..TP X2在甲状腺乳头状癌中的表达及其对甲状腺癌KAT细胞增殖能力的影响[J].广东药科大学学报,2017,33(4):551-555,5.
