高校化学工程学报2017，Vol.31Issue(4)：884-891,8.DOI:10.3969/j.issn.1003-9015.2017.04.018
重组大肠杆菌合成蛋氨酸的代谢途径优化
Metabolic Pathway Optimization of Methionine Biosynthesis Using Recombinant Escherichia Coli
摘要
Abstract
L-methionine as a sulfur-containing amino acid has important physiological functions and application values. In order to improve methionine biosynthesis, feedback repression of methionine synthesis was partially alleviated by knocking out the encoding repressor protein gene metJ and the encoding homoserine kinase gene thrB. The results show that methionine accumulation can reach to 0.18 g·L-1 using the resulting strain. Based on the known functional genes related to methionine accumulation, synthesis and metabolism of methionine was optimized concerning methionine metabolism network and transportation by knockout and overexpression techniques. Effects of key steps in methionine pathway, methionine transportation, threonine synthesis pathway and methionine recycling pathway were also investigated, and the metabolic network of E. coli was optimized. Transportation of methionine to extracellular was enhanced. Methionine concentration can reach to 0.40 g·L-1 after 24 h cultivation of the optimized strain. This work is helpful in the improvement of methionine biosynthesis.关键词
蛋氨酸/途径优化/代谢/蛋氨酸转运Key words
methionine/pathway optimization/metabolism/methionine transport分类
生物科学引用本文复制引用
高海军,杨一飞,孟青..重组大肠杆菌合成蛋氨酸的代谢途径优化[J].高校化学工程学报,2017,31(4):884-891,8.基金项目
重大科学仪器设备开发专项(2012YQ0401400802). （2012YQ0401400802）
