中医药导报2017，Vol.23Issue(15)：57-59,62,4.
响应面法优化新疆黑果小檗根皮中总生物碱提取工艺
Optimization of Extraction Process for Total Alkaloids of Berberis Heteropda Root Bark from Xinjiang by Response Surface Methodology
张婷婷 1李莉 1于文 1高宁1
作者信息
- 1. 新疆医科大学药学院,新疆乌鲁木齐830011
- 折叠
摘要
Abstract
Objective:To optimize the extraction process of total alkaloids from Xinjiang Berberis Heteropoda root bark.Methods:The content of total alkaloids was determined by Acid-Dyes-UV Spectrophotometry.The optimal process parameters were ethanol at 80 ℃ and three extraction cycles.With the content of total alkaloids as index,based on single factor experiments,the ethanol concentration,extraction time and solid-liquid ratio as influence factors,three factors and three levels Box-Behnken design of Response Surface Methodology (RSM)was adopted.Results:The optimal process parameters were as follows:extracted 2 h with 51 times 50％ ethanol at 80 ℃,three extraction cycles.Under this condition,three paralle tests were carried out,yeild of the total alkaloids could reach 4.01％,RSD％=1.12％,which accounted for 99.01％ of the predicted value.Conclusion:The optimized extraction process is stable and feasible,to provide a basis for subsequent separation and purification of the total alkaloids from Berberis Heteropoda root bark.关键词
黑果小檗根皮/总生物碱/提取工艺/响应面法Key words
Berberis heteropda root bark/total alkaloids/extraction process/response surface methodology (RSM)分类
医药卫生引用本文复制引用
张婷婷,李莉,于文,高宁..响应面法优化新疆黑果小檗根皮中总生物碱提取工艺[J].中医药导报,2017,23(15):57-59,62,4.
