解剖学杂志2017，Vol.40Issue(4)：377-381,5.DOI:10.3969/j.issn.1001-1633.2017.04.003
核转录因子-κB和缺氧诱导因子-1α在大鼠损伤脑组织中的表达变化
Expression and changes of nuclear factor-κB and hypoxia inducible factor-1α in rats with brain trauma
摘要
Abstract
Objective: To study the expression features of nuclear factor (NF)-κB,hypoxia inducible factor (HIF)-1α,and their mRNA in brain trauma with different damage time and to provide evidence for forensic dating of wounds.Methods: A total of 66 female Wistar rats were equally and randomly divided into 11 groups,namely normal control group and 10 damage groups.Rats in the 10 damage groups were put to death in post-traumatic 1,2,4,6,8,10,12,16,18 and 24 hours.The brain tissue was taken out.Pathology morphological structure changes were observed by H-E staining.Expression of NF-κB and HIF-1α and their mRNA were detected in different damage time using immunohistochemical SP method and PCR.Results: NF-κB expression rose in 1 hour after damage,reached the peak after 18 hours,from 18 to 24 hours in plateau.NF-κB positive expression in damage groups were significantly higher than that in control group except the 1 hour and 2 hour group.NF-κB mRNA level was significantly higher in damage groups than that in control group.HIF-1α was expressed at 6 hours after damage,the expression was gradually strengthened,and the strongest expression was at 24 hours after damage.HIF-1α expression was significantly higher in all damage groups than the control group,in addition to the 1,2 and 4 hour group.HIF-1α mRNA was expressed 4 hours after injury and reached the peak at 18 h,maintained till 24h.Except for the 1h and 2h injury groups,HIF-1α mRNA levels in the other groups were significantly higher than the control group,and the difference had statistical significance.Conclusion: The expression features of NF-κB,HIF-1α,and their mRNA changes with the damage time in brain trauma.The above factors may be used to speculate the forensic dating of wound.关键词
创伤性脑损伤/损伤时间/核转录因子-κB/缺氧诱导因子/大鼠Key words
traumatic brain injury/damage time/nuclear factor-κB/hypoxia inducible factor/rat引用本文复制引用
刘长海,武彦,顾捷,巩海霞,荣荣,张曙光,李志军..核转录因子-κB和缺氧诱导因子-1α在大鼠损伤脑组织中的表达变化[J].解剖学杂志,2017,40(4):377-381,5.基金项目
国家自然科学基金(81260269,81560348) （81260269,81560348）
内蒙古自治区高等学校科学技术研究项目(NJZY108) （NJZY108）
