解剖学杂志2017，Vol.40Issue(4)：421-424,4.DOI:10.3969/j.issn.1001-1633.2017.04.013
丁苯酞预处理对缺血再灌注大鼠海马神经元超微结构的影响
Effects of 3-N-butylphthalide pretreatment on ultrastructure of hippocampal neurons in rats with cerebral ischemia-reperfusion
摘要
Abstract
Objective: To explore the protective effects and mechanisms of 3-N-butylphthalide(NBP) pretreatment on hippocampal neurons of cerebral ischemia-reperfusion in rats.Methods: Forty male Sprague-Dawley (SD) rats were randomly divided into 5 groups (n=8): sham group,ischemia-reperfusion group(I/R),and low,middle,high NBP dosage group.Low,middle and high dosage group were orally administered with NBP 20,40,80mg/kg daily for 7 days before the right middle cerebral artery occlusion (MCAO).Rats in sham group and ischemia-reperfusion group were given the same volume of saline by gavage one time a day.The right MCAO focal ischemia-reperfusion rat models were established with the improved Longa-Zea method.Neurologic impairment score was evaluated with Zea-Longa`s standards at 24h after ischemia-reperfusion.The volume of cerebral infarction was identified with 2% triphenyl tetrazolium chloride (TTC) staining.Histopathological changes of hippocampal neurons were observed under H-E staining,and ultrastructural changes of hippocampal neurons were observed by transmission electron microscopy.Results: NBP pretreatment significantly reduced the volume of cerebral infarction,improved the neurological function score,protected mitochondria and mitigated the neuron damage.Conclusion: The pretreatment of 3-N-butylphthalide has prophylactic neuroprotective effect.关键词
脑缺血再灌注/丁苯酞/海马/神经元/超微结构/大鼠Key words
ischemia reperfusion/3-N-butylphthalide/hippocampus/neuron/ultrastructure/rat引用本文复制引用
王伟,李富强,刘荣志,马喻红,孔祥玉,赵淑敏..丁苯酞预处理对缺血再灌注大鼠海马神经元超微结构的影响[J].解剖学杂志,2017,40(4):421-424,4.基金项目
河北省科技厅,河北省教育厅资助课题(07276101D-46,2005227) （07276101D-46,2005227）
