解剖学杂志2017，Vol.40Issue(4)：425-428,4.DOI:10.3969/j.issn.1001-1633.2017.04.014
Ro25-6981对缺血再灌注大鼠脑室下区神经干细胞增殖的影响
Effects of Ro25-6981 on proliferation of neural stem cells in the subventricular zone of ischemia-reperfusion rats
摘要
Abstract
Objective: To investigate the role of Ro25-6981,NR2B specific antagonist,on proliferation of neural stem cells in ischemia-reperfusion rats.Methods: The model of transient brain ischemia followed by 2 hours reperfusion was induced by practicing middle cerebral artery occlusion.Male SD rats were randomly divided into sham group,ischemia-reperfusion control group and Ro25-6981 treatment group.Number of nestin and proliferating cell nuclear antigen (PCNA) positive cells stained by immunohistochemical method in the subventricular zone (SVZ).The expression of brain-derived neurotrophic factor (BDNF) was measured by Western blotting.Results: Compared to the sham group,the ischemia-reperfusion group showed the obviously increased number of nestin and PCNA positive cells in the SVZ.However,Number of nestin and PCNA positive cells in Ro25-6981 treatment group decreased compared with control group.Ischemia-reperfusion injury significantly enhanced BDNF protein expression.In contrast,NR2B specific antagonist Ro25-6081 significantly decreased BDNF protein expression.Conclusion: NR2B,the subunit of NMDA receptors,promotes proliferation of neural stem cells in the SVZ,which may be related to the regulation of BDNF expression.关键词
缺血再灌注/脑室下区/神经干细胞/增殖/N-甲基-D-天冬氨酸受体/Ro25-6981/脑源性神经生长因子Key words
ischemia-reperfusion/subventricular zone/neural stem cell/proliferation N-methyl-D-aspartic acid receptor/Ro25-6981/brain-derived neurotrophic factor/rat引用本文复制引用
唐晓洪,来青伟,李青云,李昕昱,袁蕊,王潇,胡朋,樊红彬..Ro25-6981对缺血再灌注大鼠脑室下区神经干细胞增殖的影响[J].解剖学杂志,2017,40(4):425-428,4.基金项目
江苏省卫生厅资助项目(H201319) （H201319）
