解剖学杂志2017，Vol.40Issue(4)：429-432,452,5.DOI:10.3969/j.issn.1001-1633.2017.04.015
1,8-桉叶素对背根神经节内P2X3受体介导神经病理痛的作用
Effect of 1,8-cineole on neuropathic pain mediated by P2X3 receptor in the dorsal root ganglions
摘要
Abstract
Objective: To explore the effect of 1,8-cineole on P2X3 receptors in isolated dorsal root ganglion (DRG) regulating neuropathic pain in rats.Methods: The sciatic nerve chronic constrictive injury model(CCI) of rats was established.Sprague-Dawley rats were randomly divided into sham group,chronic constriction injury (CCI) group,1,8-cineole(50mg/kg/d) treated group,1,8-cineole(100mg/kg/d) treated group and CCI+DMSO group.The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) at the 7th and 14th day after the operation were measured.P2X3 receptor expression changes in L4-5 DRG of the rats were observed by immunohistochemistry and in situ hybridization techniques.Results: 7 and 14 days after the operation,the MWT and TWL of rats in CCI group were significantly higher than that in sham group but lower significantly than that in low and high dose treatmant group.and has no difference with DMSO group.Expression of P2X3 receptor in L4-5 DRG in CCI group was significantly higher than that in sham group.Low and high dose treatment goups showed an apparent decrease compared with the CCI group,and the high dose treatment group was lower than that in low-dose treatment group.DMSO group had no difference with CCI group.Conclusion: 1,8-cineole can alleviate neuropathic pain through inhibiting the overexpression of P2X3 receptor in L4-5 DRG of CCI rats.关键词
神经病理痛/1,8-桉叶素/背根神经节/P2X3受体/大鼠Key words
neuropathic pain/1,8-cineole/dorsal root ganglion/P2X3 receptor/rat引用本文复制引用
李晴,刘怡果,王向东,郑晓波,李世城,谢晨园,杨宝林,刘曾旭..1,8-桉叶素对背根神经节内P2X3受体介导神经病理痛的作用[J].解剖学杂志,2017,40(4):429-432,452,5.基金项目
国家自然科学基金(81260190) （81260190）
江西省自然科学基金(20132BAB205023,20151BAB205022) （20132BAB205023,20151BAB205022）
江西省教育厅科学技术研究项目(GJJ13159) （GJJ13159）
