日用化学工业2017，Vol.47Issue(8)：446-451,462,7.DOI:10.13218/j.cnki.csdc.2017.08.006
酶解羊胎盘抗氧化肽的分离及在护肤品中的应用
Separation of antioxidative peptides from sheep placenta byproduct by enzymolysis and its application in cosmetics
李明 1姜惠敏 2曹光群 2杨成1
作者信息
- 1. 江南大学食品胶体与生物技术教育部重点实验室,江苏无锡214122
- 2. 江南大学化学与材料工程学院,江苏无锡214122
- 折叠
摘要
Abstract
In order to develop application of antioxidative peptides from sheep placenta byproduct by enzymolysis in cosmetics,papain was employed for the enzymolysis and DPPH radical scavenging activity of the hydrolysate was used for efficacy evaluation. The hydrolysate by papain was purified sequentially by DA201-C macroporous resin,ultrafiltration and Sephadex G-10 gel filtration. Fractions obtained from purification operations were evaluated. The sheep placenta peptide S12 displays the highest DPPH radical scavenging activity and was selected for further study. The formulas of skin care lotion and cream containing different content of S12 (0-5%)were designed,and the moisturizing and elastic effect of all skin care product samples were evaluated by MC750 skin test instrument. Results show that after continuous using the skin care lotion and cream containing 4% content of S12 for 60 d,the skin cuticula hydration of 15 volunteers increases by 27 and 31 respectively;the transepidermal water loss reduces by 12. 0 and 13. 2 g/(m2 ·h)respectively;and the skin elasticity R2 increases by 38% and 45% respectively. It is concluded that the skin care lotion and cream containing sheep placenta peptide can improve skin moisturizing and increase skin elasticity.关键词
化妆品添加剂/羊胎盘/酶解/抗氧化肽/功效评价Key words
additive of cosmetics/sheep placenta/enzymolysis/antioxidative peptide/efficacy evaluation分类
化学化工引用本文复制引用
李明,姜惠敏,曹光群,杨成..酶解羊胎盘抗氧化肽的分离及在护肤品中的应用[J].日用化学工业,2017,47(8):446-451,462,7.
