日用化学工业2017，Vol.47Issue(8)：476-480,5.DOI:10.13218/j.cnki.csdc.2017.08.011
超高效液相色谱-质谱联用法同时测定化妆品中5种禁用性激素
Simultaneous determination of five banned sex hormones in cosmetics with UPLC-MS/ MS
摘要
Abstract
A method using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC -MS / MS)was established for determination of five banned sex hormones in cosmetics. The chromatography column is selected as ACQUITY UPLC? BEN C18 column (2. 1 mm × 50 mm,1. 7 μm). The mobile phase consists of methanol-0. 1% formic acid aqueous solution (60: 40)and the flow rate is 0. 4 mL/ min. The column temperature is 30 ℃. Sample injection amount is 1 μL. The scanning method is multiple reaction monitoring (MRM)in combination with information dependent acquisition (IDA)and enhanced ion scanning (EPI). Results show that the relation between peak area and mass concentration of the detected four kinds of estrogen,including oestradiol,oestriol,oestrone and stilbestrol as well as progesterone displays good linearity with correlation coefficient higher than 0. 999. Detection limit of the method is 1-100 mg / L. The method was carried out at three spiking levels,the average recoveries are in the range of 79. 8%-115. 3%,with relative standard deviations of 1. 43%-11. 36% (n = 6).关键词
功效化妆品/雌激素/超高效液相色谱-串联质谱法/多反应监测Key words
functional cosmetics/estrogen/ultra-high performance liquid chromatography-tandem mass spectrometry/multiple reaction monitoring分类
化学工程引用本文复制引用
岳磊,李晓静..超高效液相色谱-质谱联用法同时测定化妆品中5种禁用性激素[J].日用化学工业,2017,47(8):476-480,5.基金项目
2015郑州地方高校新世纪教育教学改革及人才培养工程立项(ZZJG-B7027) （ZZJG-B7027）
