山东医药2017，Vol.57Issue(32)：21-24,4.DOI:10.3969/j.issn.1002-266X.2017.32.006
PRPS2对肺癌细胞体外生长、增殖和体内成瘤的影响及其机制
Effects of PRPS2 on lung cancer cell proliferation, tumor growth in vitro, and tumorigenic ability in vivo
摘要
Abstract
Objective To investigate the role of phosphoribosyl pyrophosphate synthetase 2 (PRPS2) in lung cancer cell proliferation, tumor growth in vitro, and tumorigenic ability in vivo.Methods Lung cancer A549 cells cultured in vitro were divided into 3 groups: the control group (scramble control shRNA lentivirus infecting cells for 12 h), Lenti-sh1 group (lentivirus Lenti-sh1 infecting cells for 12 h) and Lenti-sh2 group (lentivirus Lenti-sh2 infecting cells for 12 h).These results indicated that the mRNA and protein expression levels of PRPS2 were effectively reduced in the control group as compared with that of the other two groups (both P<0.05).These results indicated that the expression levels of PRPS2 were reduced by PRPS2 shRNA lentivirus in A549 lung cancer cells.Colony formation assay was used to detect the clone number of three groups.The cell proliferation rate was evaluated by a CCK8 assay and cell cycle was detected by flow cytometry.RT-PCR and Western blotting were used to detect the mRNA and protein expression levels of APC, β-catenin, Gsk3β in A549 cells of WNT signaling pathway.Tumors were generated by subcutaneously injecting lung cancer A549 cells from the control, Lenti-sh1 and Lenti-sh2 groups into the right forelimb axillaries of Balb/c nude mice (each group contained 6 mice).The mice were sacrificed at the 6th week to evaluate the weight and volume of tumors.Results Compared with the control group, the number of cell colonies in the Lenti-sh1and Lenti-sh2 groups were reduced (P<0.05).The absorbance value of the Lenti-sh1and Lenti-sh2 groups at 72 and 96h was lower than that of the control group (P<0.05).A significantly higher percentage of cells accumulated in the G0/G1 phase of the cell cycle in the Lenti-sh1and Lenti-sh2 groups as compared with that of the control group.However, the percentage of cells in the S and G2/M phase was lower than that of the control group (all P<0.05).The mRNA and protein levels of APC and β-catenin in the Lenti-sh1and Lenti-sh2 groups were lower than that of the control group but the mRNA and protein levels of Gsk-3β were higher than that of the control group (all P<0.05).The tumor weight and volumes of the the Lenti-sh1and Lenti-sh2 groups decreased significantly as compared with those of the control group (both P<0.05).Conclusion Silencing PRPS2 gene may suppress lung cancer growth, proliferation in vitro, and tumorigenic ability in vivo by regulating the WNT signaling pathway.关键词
肺癌/磷酸核糖焦磷酸合成酶2/细胞增殖/细胞周期/体外成瘤能力Key words
lung carcinoma/phosphoribosyl pyrophosphate synthetase 2/cell proliferation/cell cycle/tumorigenic ability in vitro分类
医药卫生引用本文复制引用
顾鸿莉,宋卫峰,王静珏,蔡讯,李琦..PRPS2对肺癌细胞体外生长、增殖和体内成瘤的影响及其机制[J].山东医药,2017,57(32):21-24,4.基金项目
上海市自然科学基金资助项目(15ZR1433600). （15ZR1433600）
