实验动物与比较医学2017，Vol.37Issue(3)：171-178,8.DOI:10.3969/j.issn.1674-5817.2017.03.001
树鼩形觉剥夺性近视模型的建立及观察
Preliminary Establishment and Research on Form Deprivation Myopia Model in Tree Shrew
摘要
Abstract
Objective To establish the adolescence and early adulthood form deprivation myopia (FDM) model in tree shrew and to observe the retinal morphology,in order to explore the role of age in the development of myopia and the effection of local retinal mechanism for FDM.Method Thirty tree shrews at age of 4 months and 5 months without congenital myopia and other eye diseases were respectively selected.All tree shrews were randomly divided into:control group,and cover group.In the cover group,right eyes served as the experimental eye,left eye as control eyes.Experimental eye were covered with handmade semi-translucent film.then measure the diopter and ?axial?length of tree shrews after being covered for 3 weeks and 6 weeks.The retinal thickness and the number of cells in each layer of retina were observed by electron microscopy after being covered for 6 weeks.Results The tree shrews were born 4 months and 5 months form deprivation after 3 weeks,hyperopia was alleviated but not statistically significant compared with control eyes,and two groups of tree shrews cover eye diopter and eye axis are different obviously compared with control eyes after 6 weeks.At deprivation period,axial continue to extend and gradually increase to myopia,and they have a good negative linear relationship.Form deprivation can lead to thinning of the retina of the tree shrew,can also lead to number decrease on photoreceptor cell layer,inner nuclear layer,and ganglion cell layer cells.Conclusion Form deprivation can induce myopia formation and retinal morphological change in adolescence and early adulthood tree shrews.关键词
树鼩/形觉剥夺性近视(FDM)/视网膜形态学Key words
Tree shrew/Form deprivation myopia (FDM)/Retinal morphology.分类
生物科学引用本文复制引用
杨冬梅,朱勤,李娜,郭立云,张晓帆,张洁莹,胡敏,代解杰..树鼩形觉剥夺性近视模型的建立及观察[J].实验动物与比较医学,2017,37(3):171-178,8.基金项目
国家自然科学基金(81560168),国家科技支撑计划项目(2014BAI01B01),云南省联合支持国家计划项目(2015GA009),云南省自然科学基金(2013FZ191),昆明医科大学重点联合专项(2014FA017),云南省卫生科技计划项目(2014NS043) （81560168）
