实验动物与比较医学2017，Vol.37Issue(3)：198-203,6.DOI:10.3969/j.issn.1674-5817.2017.03.005
ICR小鼠感染呼肠孤病毒Ⅲ型的实验研究
Research on Reovirus Ⅲ (Reo-3) Infection in ICR Mice
摘要
Abstract
Objective To establish reovirus type 3 (Reo-3) infection model of ICR mice and observe clinical syndrome,investigate virus load in target organs and serology dynamic change in infected mice.Method Animal adaption method was used to increase the virus's virulence.Systemic infection model was established with tail vein and intraperitoneally inoculation to ICR.The clinical sign was observed.The tissues and serums were harvested on 0 d before and 4 d、7 d、18 d、25 d、35 d、72 d、129 d after inoculation.Blood samples of three mice were randomly collected on 47 d、81 d、103 d.qPCR and ELISA were respectively used to detect the virus nucleic acid at target tissue and the antibody level.Results Virus virulence was increased after four times adaption trial.Mice death was observed at d7,d9,d10,d11,d16 post inoculation.The highest load of the virus was found in liver through qPCR,followed by heart,spleen,and lung.The peaks of viral load emerged from 6 to 11 d in all organs,negative results appeared at 129 d in the most organs.The earliest antibody detect time was on 18 d.The titer of antibody hit the peak on 25 d,then maintained a high level till to 129 d.Conclusion virus virulence can be enhanced significantly through mouse in-vivo inoculation,multiple organs inflammation and early death in mice can be identified by the systemic infection way of Reo-3.This experiment provides an approach to establish Reo-3 infected mice animal model and provides good experimental data for further research on Reo-3 infection mechanism.关键词
呼肠孤病毒Ⅲ型(Reo-3)/感染/临床研究/呼吸道肠道病Key words
Reovirus type 3(Reo-3)/Infection/Clinical research/Respiratory intestinal disease分类
生物科学引用本文复制引用
罗银珠,郭鹏举,黄韧,张钰,何丽芳,黄碧洪,吴瑞可,闵凡贵,潘金春,袁文,王静..ICR小鼠感染呼肠孤病毒Ⅲ型的实验研究[J].实验动物与比较医学,2017,37(3):198-203,6.基金项目
广东省科技计划项目(2014B070706006)、(2013B060400028) （2014B070706006）
