实验动物与比较医学2017，Vol.37Issue(3)：204-208,5.DOI:10.3969/j.issn.1674-5817.2017.03.006
仙台病毒核酸测序检测方法的建立
Development of Detection Method for Sendai Virus by Nucleic Acid Sequencing
摘要
Abstract
Objective To develop the method for detection of Sendai virus by nucleic acid sequencing.Methods General primers according to Sendai virus genome sequences were designed and optimized to sequencing primers.Then the best condition of creating library was established.This method was verified by nucleic acid sequencing and analysis.Results A pair of highly specific general primers was designed.The sequences are respectively 5'-GCTGCAAAACGCTGTGGG-3'and 5'-TGGRACYT-CAGAAAGAATRGG-3',and the sequencing primers were established by ligated adaptor sequences to the 5'-end of general primers.The established condition of creating library and library products were obtained by the first round amplification using general primers and the second round using sequencing primers.Sendai virus was effectively distinguish from other organisms by nucleic acid sequencing and analysis,and was precised to TianJin strain.Conclusion A detection method was developed for Sendai virus by nucleic acid sequencing,which may be as the base for microbiological detection in laboratory animals.关键词
仙台病毒(SeV)/核酸测序/检测Key words
Sendai virus/Nucleic acid sequencing/Detection分类
生物科学引用本文复制引用
张欢欢,余陈欢,戴方伟,马月,郎秋蕾,王瑜,应华忠..仙台病毒核酸测序检测方法的建立[J].实验动物与比较医学,2017,37(3):204-208,5.基金项目
浙江省卫生高层次人才,浙江省科技厅院所专项(2012F30026,2016F30001),浙江省科技厅实验动物计划项目(2016C37106) （2012F30026,2016F30001）
