实验动物与比较医学2017，Vol.37Issue(3)：214-219,6.DOI:10.3969/j.issn.1674-5817.2017.03.008
氯化钠的大鼠亚慢性毒性观察
The Sub-chronic Toxicity of Sodium Chloride in Rats
李光先 1刘香梅 1刘冬虹 1刘颖 1孙侠 1陈汉金 1郭新东 1黄宇锋1
作者信息
- 1. 广州质量监督检测研究院,广州511447
- 折叠
摘要
Abstract
Objective To explore the sub-chronic toxicity of sodium chloride on rat by oral gavage.Methods Eighty SPF SD rats were randomly divided into control,low-dose,middle-dose and highdose groups with half male and half female.The treatment groups were exposed to sodium chloride (low-dose group with 100 mg/kg,middle-dose with 500 mg/kg and high-dose group with 1 000 mg/kg) and the control group were exposed with pure water by oral gavage for continuous 90 days.Clinical observations,daily diet consumption and body weight of rats in each group were recorded during exposure.After exposure completed,the rats were anesthetized and blood samples were collected from abdominal aorta to test the routine blood indexes,serum biochemistry and plasma electrolytes.The main organs were dissected and weighed to calculate the organ coefficients.Histopathology changes of main organs were observed by microscopy.Results There were no difference in body weight,blood routine index,daily ration and organ coefficients between all groups (P＞0.05).Serum biochemistry examination showed that,compared with the control group,the ALT and AST were decreased (P＜0.05) while the TBIL and calcium content were increased (P＜0.05) in middle-dose and high-dose groups.In the pathological examination of rats,pathological changes of spongy degeneration,congestion,necrosis and infiltration of liver were found in some rats in middle-dose and high-dose groups.And some male rats in middle-dose and high-dose groups also found renal?tubular calcification,thymic hemorrhage and bleeding tendency of lymph nodes.No changes of biological significance were found in the serum biochemistry examination in the control and low-dose group.No pathological lesion can been seen in the control and low-dose group.Conclusion The sub-chronic toxicity of sodium chloride by oral administration can cause blood circulatory disorder and renal deposition of calcium salt in male rat.关键词
氯化钠/大鼠/亚慢性毒性Key words
Sodium chloride/Rats/Sub-chronic toxicity分类
生物科学引用本文复制引用
李光先,刘香梅,刘冬虹,刘颖,孙侠,陈汉金,郭新东,黄宇锋..氯化钠的大鼠亚慢性毒性观察[J].实验动物与比较医学,2017,37(3):214-219,6.
