实验动物与比较医学2017，Vol.37Issue(3)：231-239,9.DOI:10.3969/j.issn.1674-5817.2017.03.013
SPF鸡不同生长阶段粪便菌群组成及多样性研究
Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages
摘要
Abstract
Objective To provide the composition and diversity of intestinal flora at different growth stages of SPF chickens.Methods The feces of SPF chickens at 10 d,12 weeks,23 weeks and 52 weeks old were collected.The two hypervariable regions (V3-V4) of the 16S rRNA of the samples were sequenced using the Illumina HiSeq 2500 platform,and the alpha diversity,beta diversity and fecal community characteristics were analyzed.Results The fecal flora sample at four time points had similar high abundance and good uniformity and there was no significant difference in species diversity between the two groups (P＞0.05).More than 95％ of abundance were found in Firmicutes,Proteobacteria and Bacteroidetes;Lactobacillus,Streptococcus,Bacteroides,and Enterococcus were dominant species,and the abundance of different periods were significantly different (P＜0.05).Conclusions The feces flora of SPF chiken at different growth stages are diverse,and the dominant bacteria are mainly beneficial bacteria.The experimental results are expected to provide the basis for assessing the quality,health monitoring and safety evaluation of SPF chickens.关键词
SPF鸡/不同生长阶段/粪便菌群/16s rRNA/Illumina HiSeq 2500高通量测序Key words
SPF chicken/Different growth stages/Feces microbiota/16s rRNA/Illumina HiSeq 2500 platform分类
生物科学引用本文复制引用
周妍,刁晨曦,张圆圆,于海波,陆涛峰,赵丽丽,陈洪岩..SPF鸡不同生长阶段粪便菌群组成及多样性研究[J].实验动物与比较医学,2017,37(3):231-239,9.基金项目
国家科技支撑计划(2015BAI07B02-02) （2015BAI07B02-02）
中国农业科学院基本科研业务费专项(Y2016PT41) （Y2016PT41）
国家自然科学基金(31601974) （31601974）
