实验动物与比较医学2017，Vol.37Issue(3)：244-248,5.DOI:10.3969/j.issn.1674-5817.2017.03.015
miR-200b-3p和miR-200b-5p在马立克氏病抗性与易感SPF鸡法氏囊组织的差异表达分析
Differential Expression Analysis of miR-200b-3p and miR-200b-5p in Marek's Disease Resistant and Susceptible SPF Chickens
摘要
Abstract
Objective To validate high throughput sequencing results by fluorescence quantitative PCR technique and analyze the expression of miR-200b-3p and miR-200b-5p in bursa,and explore possible molecular mechanism associated with Marek's disease (MD) resistance/susceptibility.Methods Through optimization,the ideal amplification conditions and reaction system of real-time fluorescence quantitative PCR were identified.Results miR-200b-3p and miR-200b-5p fluorescence quantitative PCR results and high throughput sequencing results were consistent in down-regulation,but not significant difference.miR-200b-3p and miR-200b-5p two homologous miRNA showed similar expression after infected by Marek's disease virus (MDV).Conclusion Although the difference is not consistent with the results of significant analysis,miR-200b-3p and miR-200b-5p may be associated with resistance / susceptibility of MD.关键词
miR-200b/马立克氏病(MD)/抗性/易感性/高通量测序/荧光定量PCRKey words
MiR-200b/Marek's disease (MD)/Resistance/susceptibility/High throughput sequencing/Fluorescence quantitative PCR分类
生物科学引用本文复制引用
王瑞琪,廉传江,易诚,韩凌霞,杨春文,陈洪岩..miR-200b-3p和miR-200b-5p在马立克氏病抗性与易感SPF鸡法氏囊组织的差异表达分析[J].实验动物与比较医学,2017,37(3):244-248,5.基金项目
国家自然科学基金项目(31500997) （31500997）
黑龙江省科技计划项目(PC13S04) （PC13S04）
