生态学杂志2017，Vol.36Issue(8)：2217-2223,7.DOI:10.13292/j.1000-4890.201708.004
风速对转基因棉花基因漂移的影响
The influences of wind speed on pollen-mediated gene flow from transgenic cotton
摘要
Abstract
The genetically modified (GM) crops have been globally commercially cultivated for 20 years,and public concerns are persistent overheating about ecological risk of gene flow.This study investigated the pollen-mediated gene flow (PGF) frequency of Cry1Ac from transgenic cotton SGK321 (CrylAc/CpTI) to non-transgenic cottons Shiyuan 321 and Zhongmian 35 under three wind conditions (0.54,0.92 and 1.27 m · s-1) in greenhouses by PCR and Bt protein assays.Our results revealed that PGF varied depending on the wind speed when Shiyuan 321 was the pollen receptor,and was the highest under low speed wind and the lowest under high speed wind.With Zhongmian 35 as pollen receptor,PGF was very low,and not dependent on the wind speed.PGF depended on the variety of pollen receptors,and total PGF to pollen receptor of Shiyuan 321 (GM parental line) (10.63％) was higher than that of Zhongmian 35 (2.50％).PGF declined exponentially with increasing distance between GM cotton and non-GM receptors.Our research provided some reference for ecological safety assessment of wind-mediated gene flow.关键词
基因漂移/转基因棉花/生态安全/花粉授体/定性PCRKey words
gene flow/transgenic cotton/ecological safety/pollen receptor/qualitative PCR引用本文复制引用
闫硕,刘小侠,朱家林,朱威龙,秦萌,刘慧,赵守歧,王璐,张璟,张青文..风速对转基因棉花基因漂移的影响[J].生态学杂志,2017,36(8):2217-2223,7.基金项目
转基因棉花环境安全评价技术项目(2016ZX08011002)资助. （2016ZX08011002）
