天津科技大学学报2017，Vol.32Issue(3)：16-22,7.DOI:10.13364/j.issn.1672-6510.20160023
类志贺邻单胞菌噬菌体 ФP4-7裂解酶Gp2的表达及活性测定
Characterization and Activity Determination of Gp2 Endolysin from Plesiomonas shigelloides Phage ФP4-7
摘要
Abstract
The emergence of multi-drug resistant pathogens has brought difficulties to clinical treatments.The endolysin encoded phage could lyse pathogenic microorganisms,and as an important bactericidal agent,it is a potential alternative to antibiotics.In this study,gp2 gene of Plesiomonas shigelloides phage ФP4-7,was amplified and cloned into pQE30 plas-mid.The recombined protein was purified by Ni-NTA agarose resin.The optimal acidity of recombined endolysin was pH 9.After treatment at 40℃ for 15 min,it retained approximately 56.4% of its lytic activity.The GP2 endolysin could cleave 5G- strains(Pseudomonas aeruginosa ,Escherichia coli ,Serratia marcescens ,Morganella morganii ,Citrobacter freundii)and 3G+ strains(Staphylococcus aureus,Bacillus subtilis,Bacillus licheniformis).In combination with EDTA, LAB35,or SDS,respectively,Gp2 displayed synergetic antimicrobial activities to both Pseudomonas aeruginosa and Bacil-lus subtilis.Our results indicate that Gp2 endolysin is effective in killing some pathogens and might be used as an antibacte-rial agent.关键词
噬菌体裂解酶/类志贺邻单胞菌噬菌体ΦP4-7/蛋白表达/杀菌活性Key words
phage endolysin/Plesiomonas shigelloides phage ФP4-7/expression of protein/antibacterial activity分类
生物科学引用本文复制引用
何洋,荆兆元,杨洪江..类志贺邻单胞菌噬菌体 ФP4-7裂解酶Gp2的表达及活性测定[J].天津科技大学学报,2017,32(3):16-22,7.基金项目
国家自然科学基金资助项目(31370205,30970114) （31370205,30970114）
