天津科技大学学报2017，Vol.32Issue(3)：23-28,6.DOI:10.13364/j.issn.1672-6510.20160054
诱导子对蜀葵悬浮细胞、不定根培养的影响
Effects of Elicitors on Uronic Acid and the Total Flavonoid Content in Alcea rosea Suspension Cells and Adventitious Root
摘要
Abstract
In order to explore the influence of elicitors on secondary metabolites of Alcea rosea cells,methyl jasmonate, salicylic acid and yeast extracts were used to treat Alcea rosea suspension cells and adventitious roots.The results showed that methyl jasmonate and salicylic acid inhibited the growth of suspension cells and adventitious roots,but low concentra-tion of yeast extracts were beneficial to their growth.While methyl jasmonate had no effect on the uronic acid content in both cultures,salicylic acid and yeast extracts improved their uronic acid contents,and yeast extracts functioned better.In addi-tion,the total flavonoid contents were effectively improved by these elicitors,and methyl jasmonate was the best,followed by salicylic acid.These results concluded that during Alcea rosea suspension cells and adventitious roots culturing in vitro, all the three elicitors have effect on cultures and the secondary metabolites in some degree,but the best was yeast extracts, followed by salicylic acid.关键词
蜀葵/悬浮细胞/不定根/诱导子/糖醛酸/总黄酮Key words
Alcea rosea/suspension cells/adventitious root/elicitors/uronic acid/total flavonoids分类
生物科学引用本文复制引用
别振宇,李兴林,汪珊英..诱导子对蜀葵悬浮细胞、不定根培养的影响[J].天津科技大学学报,2017,32(3):23-28,6.基金项目
科技部星火计划资助项目(2012GA610011) （2012GA610011）
国家自然科学基金资助项目(U1332123) （U1332123）
