天津科技大学学报2017，Vol.32Issue(4)：24-29,35,7.DOI:10.13364/j.issn.1672-6510.20160018
大麻纤维微生物脱胶菌株的筛选、鉴定及其应用
Screening and Identifying Strains for Microbial Degumming of Hemp and their Application
摘要
Abstract
For screening and obtaining microbial strains suitable for degumming of hemp,two fine strains were selected from the degumming liquid through preliminary screening using selection plates and then rescreening again usingshake flask.The residual gum rate of degummed hemp was taken as the index.Two effective strains for degumming hemp were obtained,which were identified as Bacillus subtilis through sequence analysis of 16,S rRNA and morphology observa-tion.The raw hemp was put into a shaker at 37,℃ for 4,days with the strains;correspondingly the residual gum rate was decreased from 50%, to 13%, and the cellulose content increased from 50%, to 82%,.SEM shows that the fiber was com-pletely divergent and in bright white color without obvious damage.These results indicate that the screened strains possess the potential to be applied to industry and the research has provided important parameters for degumming hemp by microor-ganism.关键词
大麻/微生物脱胶/枯草芽胞杆菌/残胶率Key words
hemp/microbial degumming/Bacillus subtilis/residual gum rate分类
生物科学引用本文复制引用
杨田,随树珍,刘逸寒,黎明..大麻纤维微生物脱胶菌株的筛选、鉴定及其应用[J].天津科技大学学报,2017,32(4):24-29,35,7.基金项目
国家自然科学基金资助项目(21176190) （21176190）
天津市科技支撑计划资助项目(11ZCKFSY00900) （11ZCKFSY00900）
