天津科技大学学报2017，Vol.32Issue(4)：30-35,6.DOI:10.13364/j.issn.1672-6510.20160080
GSNO通过亚硝酰化抑制Myocardin诱导的心肌肥厚
GSNO Inhibiting Myocardin Induced Cardiomyocyte Hypertrophy Via S-nitrosylation
摘要
Abstract
Myocardin can induce cardiomyocyte hupertrophy and nitric oxide can protect cardiac tissue,but the effectof NO on cardiomyocyte hypertrophy remains unclear.First,in this research,NO fluorescent probe was used to detect the level of NO in H9c2 cells.The results show that the NO level was significantly increased after S-Nitrosoglutathione(GSNO) treatment.Myocardin could be S-nitrosylated and modified when the cells were treated with 200,μmol/L GSNO,and inhib-itedα-MHC expression in both mRNA and protein.Finally,overexpressing Myocardin can activate the transcription of GSNO reductase(GSNOR)expression and reduce the amount of intracellular NO.In conclusion,S-nitrosylation can inhibite hypertrophy induced by Myocardin.Myocardin can activate GSNOR transcription and indirectly inhibit GSNO's effect on Myocardin.关键词
亚硝酰化/Myocardin/GSNO/GSNORKey words
S-nitrosylation/Myocardin/GSNO/GSNOR分类
生物科学引用本文复制引用
郑德亮,廖兴华,魏兆强,杜福,周浩,张同存..GSNO通过亚硝酰化抑制Myocardin诱导的心肌肥厚[J].天津科技大学学报,2017,32(4):30-35,6.基金项目
国家自然科学基金资助项目(31171303) （31171303）
