现代妇产科进展2017，Vol.26Issue(8)：569-573,5.DOI:10.13283/j.cnki.xdfckjz.2017.08.002
脐血流异常对选择性宫内生长受限代谢组学影响的初步探讨
Preliminary analysis of metabonomics in selective intrauterine growth restriction with abnormal umbilical artery Doppler flow
摘要
Abstract
Objective:To analyze the correlation between umbilical artery (UA) Doppler flow and selective intrauterine growth restriction (sIUGR) cord blood metabolomics.Method:Totally 6 sIUGR with abnormal UA Doppler flow and 3 normal controls were enrolled.Gas chromatography-Time-of-flight mass spectrometry (GC-TOF/MS) was performed on cord blood.Pattern recognition by multivariate analysis including principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) was applied.Several metabolites different between two groups were identified by variable importance in the projection values and Student''s t-test,and statistically significant metabolites were subjected to metabolic pathways analysis.Result:Seventeen metabolites were identified.Five variables,including glutamic acid,oxalic acid,cycloleucine,N-(2-hydroxyethyl)-iminodiacetic acid,L-kynurenine,increased significantly,while aspartic acid,N-acetyl-L-aspartic acid,lactic acid,L-Threose,tyrosine,glycine,fumaric acid,beta-Mannosylglycerate,beta-Alanine,linoleic acid,palmitic acid and maleamate were significantly decreased in sIUGR with abnormal UA Doppler flow.Conclusion:GC-TOF/MS can be used to characterize changes in metabolomics of sIUGR with abnormal UA Doppler flow,our data also suggest that metabolic pathways including lipid,carbohydrate,amino acid,energy,nucleotide,as well as cofactors and vitamins metabolism changed.关键词
单绒毛膜双胎/选择性宫内生长受限/脐动脉多普勒血流/代谢组学/气相色谱-飞行时间质谱Key words
Monochorionic diamnionic/Selective intrauterine growth restriction(sIUGR)/Umbilical artery Doppler flow/Metabolomics/Gas chromatography-Time-of-flight mass spectrometry(GC-TOF/MS)分类
医药卫生引用本文复制引用
张晓威,李璐瑶,史晓明,王颖,魏瑗,原鹏波,赵扬玉..脐血流异常对选择性宫内生长受限代谢组学影响的初步探讨[J].现代妇产科进展,2017,26(8):569-573,5.基金项目
"生殖健康及重大出生缺陷防控研究"重点专项(项目编号2016YFC1000400,课题编号2016YFC1000408) （项目编号2016YFC1000400,课题编号2016YFC1000408）
