现代妇产科进展2017，Vol.26Issue(8)：578-582,587,6.DOI:10.13283/j.cnki.xdfckjz.2017.08.004
miR-101通过调节p-ERK1/2蛋白表达对SKOV3/DDP细胞行为活性的影响
The effect of miR-101 on the behavior activity of SKOV3/DDP cells by regulating the expression of p-ERK1/2 protein
摘要
Abstract
Objective:To explore the effect of miR-101 on the behavioral activity of the chemo-resistant epithelial ovarian cancer cells SKOV3/DDP and the related mechanism.Methods:SKOV3/DDP cells were up-regulated the expression of miR-101.RT-PCR assay was applied for the expression of miR-101 mRNA,and the cisplatin sensitivity of SKOV3/DDP cells were measured by CCK-8.The migration ability of SKOV3/DDP cells were tested by scratch assay and the invasion ability of SKOV3/DDP cells were tested by Transwell assay.The apoptosis of SKOV3/DDP cells were detected by V-FITC/ PI Annexin flow cytometry,the expressions of p-ERK1/2,Vimntin and E-cadherin protein were detected by Western blot.Results:Compared with the control groups,the expression level of miR-101 mRNA in miR-101 group was increased significantly (P<0.05),the cisplatin IC50 of cisplatin was significantly decreased versus the control cells (P<0.05),and the wound healing distance was significantly broadened (P<0.05),and the number of membrane cells was significantly decreased (P<0.05).The apoptosis rate of miR-101 plasmid group was much higher than that of control groups (P<0.05).The expression levels of p-ERK1/2 and Vimntin protein in miR-101 group were significant lower than those in control groups (P<0.05),while the E-cadherin and Caspase-3 expressions were obviously induced.Conclusion:Overexpression of miR-101 could inhibit the expression of p-ERK1/2 protein and inhibit the biological activity of SKOV3/DDP cells,and promote the apoptosis of SKOV3/DDP cell.关键词
miR-101/迁移/侵袭/细胞凋亡/p-ERK1/2Key words
miRNA-101/Migration/Invasion/Apoptosis/p-ERK1/2分类
医药卫生引用本文复制引用
代娟,韩萍,王秀娟,曹燕花,杨莉,肖远革..miR-101通过调节p-ERK1/2蛋白表达对SKOV3/DDP细胞行为活性的影响[J].现代妇产科进展,2017,26(8):578-582,587,6.基金项目
河北省临床医学优秀人才培养计划(No:310103020704) （No:310103020704）
