现代妇产科进展2017，Vol.26Issue(8)：583-587,5.DOI:10.13283/j.cnki.xdfckjz.2017.08.005
IGF1R在卵巢子宫内膜异位囊肿中的表达及意义
Expression and significance of IGF1R in ovarian endometriosis
摘要
Abstract
Objective:To detect the expression of insulin-like growth factor-I receptor (IGF1R) in normal endometrium and ovarian endometriotic tissues and to explore its relationship with EMs.Methods:Immunohistochemistry and western blot assay were adopted to examine the distribution and expression of IGF1R in 50 normal endometrium and 55 ectopic endomtrium tissues.Grouping the tissues depends on their menstruation cycle.The proliferation of endometrium cells was dectected by EdU after siRNA targeting IGF1R being transfected.Results:(1)IHC staining results showed that IGF1R was expressed in glandular epithelial cytoplasma and membrane of normal endometrium and ectopic endometrium.There was statistic significant difference between normal endometrium and ectopic endometrium (P=0.014).In proliferative stage tissues of two groups,there was no significant difference.While in secretory phase,the expression of IGF1R was higher in ectopic endmetrium than that in endometrium (P=0.036).In normal endometrium,the expression of IGF1R was higher in secretory phase than in the proliferative stage (P<0.001).(2)The results of western blot was accordance with IHC staining,the expression of IGF1R was higher in ectopic tissues than normal endometrium.There was a significant difference between two groups especially in the secretory phase tissues (P=0.023).(3)The proliferation of the primary endometrium cells was decreased significantly after inhibiting the expression of IGF1R (P=0.014).Conclusion:The expression of IGF1R in the ovarian endometriotic tissues was higher than the normal endometrium,it suggested that IGF1R may be involved in the development of endometriosis through inducing the proliferation of the ectopic endometrium.关键词
IGF1R/子宫内膜异位症/反义寡核苷酸/细胞增殖Key words
IGF1R/Endometriosis/siRNA/Proliferation分类
医药卫生引用本文复制引用
李莎,吴章颖,张淳,张庆华,王世宣,汪雯雯..IGF1R在卵巢子宫内膜异位囊肿中的表达及意义[J].现代妇产科进展,2017,26(8):583-587,5.基金项目
中央高校基本科研业务费专项资金资助(No:2016YXMS115) （No:2016YXMS115）
