现代妇产科进展2017，Vol.26Issue(8)：592-594,598,4.DOI:10.13283/j.cnki.xdfckjz.2017.08.007
SD大鼠妊娠期糖尿病对雌性子代糖代谢的影响及其机制研究
Effect of maternal gestational diabetes mellitus on glycometabolism in female offspring in SD rat
詹艳萍 1张勇 1张泽林1
作者信息
- 1. 三峡大学第一临床医学院&宜昌市中心人民医院,宜昌 443003
- 折叠
摘要
Abstract
Objective:To investigate the effect of maternal gestational diabetes mellitus on glycometabolism of female offspring in SD rat.Methods:Sprague-Dawley rats (8-week-old) were used in this study.Maternal gestational diabetes mellitus was induced by a single injection of streptozotocin (STZ) on the first day of gestation in Sprague-Dawley rats.Control rats were injected with citrate buffer.Serum insulin and lipids were determined by enzyme linked immunosorbent assay (ELISA).Islet area were evaluated through haematoxylin and eosin staining.Glucose and insulin tolerance tests (GTT and ITT) and insulin releasing test (IRT) were performed.microRNAs expression were evaluated through qPCR in islet.Results:Compared with offspring control group,diabetes offspring group showed more slight impairment in GTT and ITT.In addition,they showed bigger islet throughout the study.The miRNA-130a expression was decreased in diabetes offspring group.Conclusion:Maternal gestational diabetes mellitus may be a high-risk factor of glycometabolism disturbance in female offspring.关键词
妊娠期糖尿病/大鼠/子代/糖代谢/microRNAsKey words
Gestational diabetes mellitus/Rat/Offspring/Glycometabolism/MicroRNAs分类
医药卫生引用本文复制引用
詹艳萍,张勇,张泽林..SD大鼠妊娠期糖尿病对雌性子代糖代谢的影响及其机制研究[J].现代妇产科进展,2017,26(8):592-594,598,4.
