中国组织工程研究2017，Vol.21Issue(24)：3773-3777,5.DOI:10.3969/j.issn.2095-4344.2017.24.001
骨桥蛋白可促进骨关节炎软骨细胞增殖
Osteopontin promotes chondrocyte proliferation in osteoarthritis
摘要
Abstract
BACKGROUND:Osteopontin, a kind of extracellular matrix glycoprotein, has been found to participate in synthesis and catabolism of osteoarthritic chondrocyte extracellular matrix. However, the effect of osteopontin on the proliferation ability of osteoarthritic chondrocytes is little reported. OBJECTIVE:To investigate the effect of osteopontin on the chondrocyte proliferation in human knee osteoarthritis. METHODS: Cartilage samples were obtained from the patients with knee osteoarthritis undergoing knee arthroplasty at the Xiangya Hospital from January 2012 to June 2012. The chondrocytes were isolated and cultured, and then divided into four groups: blank control, osteopontin, Con-shRNA and osteopontin-shRNA groups. The cell proliferation was detected by MTT and BrdU assays. RESULTS AND CONCLUSION: After transfection of osteopontin-shRNA lentivirus, the infection rate was up to 80%. Compared with the blank control group, osteopontin group showed a significant increase in the absorbance value of osteoarthritic chondrocytes, while after osteopontin-shRNA lentivirus transfection, the absorbance value was significantly decreased (bothP < 0.05). Additionally, after osteopontin-shRNA transfection, the expression level of osteopontin was significantly downregulated (P < 0.05). To conclude, osteopontin can promote the proliferation of osteoarthritic chondrocytes, which is considered as a new treatment target for osteoarthritis.关键词
组织构建/软骨细胞/骨桥蛋白/骨关节炎/细胞增殖/RNA干扰/慢病毒转染/MTT/BrdU分类
医药卫生引用本文复制引用
姜未,林博文,张轩,王华,高曙光,张方杰..骨桥蛋白可促进骨关节炎软骨细胞增殖[J].中国组织工程研究,2017,21(24):3773-3777,5.基金项目
深圳市科技创新项目(JCYJ20150403101028191) Funding:the Science and Technology Innovation Foundation of Shenzhen, No. JCYJ20150403101028191 （JCYJ20150403101028191）
