中国组织工程研究2017，Vol.21Issue(24)：3784-3789,6.DOI:10.3969/j.issn.2095-4344.2017.24.003
人腰椎小关节软骨不同染色方法的差异比较
Different staining methods used for human lumbar facet joint cartilage: a comparative study
摘要
Abstract
BACKGROUND:With the development of modern pathological techniques, the misdiagnosis rate has been reduced remarkably, but special stains are still the most important method for pathological diagnosis. OBJECTIVE:To compare the advantages and disadvantages of different special stains used for observing the structure of human lumbar facet joints. METHODS:The specimens of facet joint cartilage at L4/5 level were collected from patients undergoing lumbar surgery, and then stained with hematoxylin-eosin, safranin O, toluidine blue, Masson, and saranin-O-fast green for structure observation. RESULTS AND CONCLUSION:The structure of the articular cartilage could be observed clearly through hematoxylin-eosin, toluidine blue, and saranin-O-fast green staining. The cartilage surface, tidemark, and subchondral bone were shown by the hematoxylin-eosin staining, with the presence of violet chondrocyte nuclei. Safranin-O-fast green staining showed the four layers of the cartilage clearly, including the shallow layer (cartilage surface), middle layer (spherical cells arranged in disorder), columnar cell layer (large and multinucleated chondrocytes arranged neatly), tidemark, subchondral bone layer; and the cartilage matrix was reddish uniformly, the subchondral bone was green, and the cartilage and bone tissue showed a striking contrast. The cartilage structure was unclear in toluidine blue staining, with clear nuclei and almost no coloring cytoplasm, but the matrix appeared with slight purplish blue. Safranin O staining showed that the cartilage was red, which had no obvious boundary with the cartilage matrix, and chondrocytes were stained lightly. Masson staining showed clear collagen fibers, but the structures of the cartilage and subchondral were obscure. To conclude, safranin-O-fast green staining can achieve the best results, followed by hematoxylin-eosin staining and Masson staining in turn.关键词
组织构建/软骨组织工程/腰椎小关节/关节软骨/特殊染色/苏木精-伊红染色/番红O染色/甲苯胺蓝染色/Masson染色/番红-固绿染色分类
医药卫生引用本文复制引用
黄磊涛,赖琦,李帆,毕海迪,吴霞,刘序强,张斌,戴闽..人腰椎小关节软骨不同染色方法的差异比较[J].中国组织工程研究,2017,21(24):3784-3789,6.基金项目
南昌大学研究生创新专项资金(cx2016390)Funding: the Innovation Foundation for the Graduate in Nanchang University, No. cx2016390 （cx2016390）
