激素联合去卵巢诱导大鼠股骨骨质疏松复合模型的建立
Establishment of a rat osteoporotic model by ovariectomy combined with glucocorticoid exposure
摘要
Abstract
BACKGROUND:There are many postmenopausal women taking hormone, which leads to much loss of bone mass, further inducing fragility fractures. The studies on the hormone exposure combined with ovariectomy-induced osteoporotic model are still immature, and the related molecular mechanism remains unclear. OBJECTIVE: To establish the rat osteoporotic model induced by ovariectomy combined with glucocorticoid exposure and to explore the underlying molecular mechanism. METHODS: Thirty 3-month-old female Sprague-Dawley rats were randomly divided into blank control, sham and model groups (n=10 per group). The rats in the blank control group received no intervention; rats in the sham group were clipped off a little of coeliac adipose tissue; the model rats received bilateral ovariectomy and 4-week administration of glucocorticoid. RESULTS AND CONCLUSION:At 4 weeks after modeling, compared with blank control and sham groups, the model group showed significantly lower bone mineral density of the femur, number of bone trabeculae and bone volume/total volume, and significantly wider bone trabecular spacing. Additionally, the model group revealed the damaged bone trabecular structure and thiner cortical bone. The expression level of Runx2 was downregulated whereas both collagen type 1α1 and peroxisome proliferators activated receptor γ mRNA were upregulated in the model group. These findings suggest that ovariectomized rats exposed to glucocorticoid rapidly develop femur osteoporosis, maybe by downregulating the expression of Runx2, as well as upregualting collagen type 1α1 and peroxisome proliferators activatedreceptor γ mRNA.关键词
组织构建/骨组织工程/糖皮质激素/去卵巢/骨质疏松症/复合模型/机制/绝经后骨质疏松症/Runx2/Ⅰ型胶原α1/过氧化物酶体增殖物激活受体γ/国家自然科学基金分类
医药卫生引用本文复制引用
张玉卓,杨志东,姚珍松,梁德,沈耿杨,张志达,招文华,黄锦菁,余翔,丘婷,卢永锵,詹玫琦..激素联合去卵巢诱导大鼠股骨骨质疏松复合模型的建立[J].中国组织工程研究,2017,21(24):3858-3863,6.基金项目
国家自然科学基金(81503591、81674000) （81503591、81674000）
广东省自然科学基金(2014A030310082) （2014A030310082）
广东省科学技术厅-广东省中医药科学院联合科研项目(2016A030313645,2014A020221021) （2016A030313645,2014A020221021）
广州中医药大学第一临床医学院优秀博士学位论文培育项目(YB201501 ,YB201602) （YB201501 ,YB201602）
广州中医药大学第一附属医院创新强院项目(2015QN03) Funding:the National Natural Science Foundation of China, No. 81503591 and 81674000 （2015QN03）
the Natural Science Foundation of Guangdong Province, No. 2014A030310082 （）
the Research Project of Guangdong Provincial Department of Science and Technology-Guangdong Academy of Chinese Medicine, No. 2016A030313645 and 2014A020221021 （）
the Excellent Doctoral Dissertation Training Project of the First Clinical Medical School of Guangzhou University of Chinese Medicine, No. YB201501 and YB201602 （）
the Innovation and Hospital Improvement Project of the First Affiliated Hospital of Guangzhou University of Chinese Medicine, No. 2015QN03 （）
