西南林业大学学报2017，Vol.37Issue(4)：1-7,7.DOI:10.11929/j.issn.2095-1914.2017.04.001
木豆抗旱相关基因CcGST1克隆与表达分析
Cloning and Expression Analysis of the Glutathione S-transferase Gene ( CcGST1) Related to Drought from Cajanus cajan
摘要
Abstract
One glutathione S-transferase ( GST) gene associated with the drought responsiveness was cloned from Cajanus cajan to explore the stress resistance mechanism by rapid amplification of cDNA ends. It was designat-ed CcGST1 and contained an open reading frame of 669 bp, which encodes 223 amino acid residues. It also con-tained conserved glutathione S-transferase N- and C-terminal domain. Phylogenetic analysis revealed that CcGST1 was closely related to GST from other legume plants, such as Glycine max and Phaseolus aculifolius. Subcellular lo-calization analysis indicated that CcGST1 was targeted to the chloroplast. By fluorescent quantitative real-time PCR, CcGST1 mRNA transcripts were detectable in all tissues, with the highest transcriptional level found in the root. Furthermore, the expression of CcGST1 transcript was markedly induced by drought stimulation. The result suggested that the CcGST1 might be involved in drought response in C. cajan.关键词
木豆/谷胱甘肽转移酶/克隆/基因/生物信息学/表达分析Key words
Cajanus cajan/glutathione S-transferase (GST)/colone/gene/bioinformatics/expression analysis分类
农业科技引用本文复制引用
乔光,文晓鹏,洪怡..木豆抗旱相关基因CcGST1克隆与表达分析[J].西南林业大学学报,2017,37(4):1-7,7.基金项目
国家自然科学基金项目 (30860224) 资助 （30860224）
贵州省农业攻关项目 (20092076) 资助. （20092076）
