西南林业大学学报2017,Vol.37Issue(4):8-14,7.DOI:10.11929/j.issn.2095-1914.2017.04.002
思茅松EST-SSR标记在几种松属植物中的通用性分析
The Transferability Analysis of Pinus kesiya var. langbianensis EST-SSR Markers to Several Pine Species
摘要
Abstract
The transferability of 18 Pinus kesiya var. langbianensis EST-SSR markers to related species was studied. The transferability percentages of selected primers to P. massoniana, P. densata, P. tabuliformis and P. yunnanensis were 88. 89%. The percentage of polymorphism of 18 EST-SSR markers to P. massoniana, P. densata, P. tabuliformis and P. yunnanensis were 81. 25%, 81. 25%, 75. 00%and 93. 75%, respectively. These results indicated that it was feasible to develop SSR markers from P. kesiya var. langbianensis transcriptome se-quences. P. kesiya var. langbianensis EST-SSR markers was highly transferable and polymorphic to its related spe-cies. These markers can be used to analyze the genetic relationship between the P. kesiya var. langbianensis and its related species.关键词
思茅松/松属/EST-SSR标记/通用性/遗传Key words
Pinus kesiya var. langbianensis/Pinus/EST-SSR markers/transferability/heredity分类
农业科技引用本文复制引用
蔡年辉,许玉兰,王大玮,唐红燕,李根前..思茅松EST-SSR标记在几种松属植物中的通用性分析[J].西南林业大学学报,2017,37(4):8-14,7.基金项目
西南林业大学科研启动基金项目 (01102-111436) 资助 (01102-111436)
国家自然科学基金项目 (31360189、 31500536) 资助 (31360189、 31500536)
云南省林学一流学科建设经费资助. ()