眼科新进展2017，Vol.37Issue(8)：701-704,708,5.DOI:10.13389/j.cnki.rao.2017.0178
N-乙酰-5-羟色胺(NAS)对视网膜缺血再灌注损伤(RIRI)大鼠视网膜活性Caspase-3、Bcl-2、Bax表达的影响
Effects of N-acetylserotonin on expression of active caspase-3, Bcl-2 and Bax protein in rat retina after ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effects of N-acetylserotonin (N-AS) on the expression of active caspase-3,Bcl-2 and Bax in rat retinas induced by retinal ischemia-reperfusion injury (RIRI).Methods Adult male Sprague-Dawley rats were randomly divided into the normal control group (6 cases),RIRI group (30 cases) and NAS group (30 cases),RIRI models in NAS group were established after giving NAS,the groups were sub-divided into 6 hours,12 hours,24 hours,48 hours and 72 hours group based on the time of RIRI.Morphologic changes were evaluated by HE staining.The expression of active caspase-3,Bcl-2 and Bax protein in the retina of rats was detected by immunohistochemistry.Results HE staining showed that the retinal structure in the normal control group was clear,and the cells in each layer were tightly packed;Each layer of retina was edema in the RIRI group after 6 hours and 12 hours,the edema gradually alleviated after 24 hours,the ganglion cells decreased gradually,the distribution was in disorder,with the prolongation of time,the retinal ganglion cells were defected;drug group of as Compared with RIRI group,the cell edema in the NAS group at 6 hours and 12 hours were obvious reduced,the cells in 24 hours,48 hours,72 hours group arranged regularly,the loss number of ganglion cells were reduced.The number of active caspase-3 positive cells in RIRI group increased at 6 hours after peffusion,the number was (561.15 ±37.19) cell ·mm-2,and reached the high level at 24 hours,the number was (1522.61 ±84.36) cell · mm-2,and then decreased gradually.The number of active caspase-3 positive cells in NAS group was significantly lower than that in RIRI group,the difference was statistically significant (all P ＜ 0.05).The expression of Bcl-2 positive cells in RIRI group began to decrease after 6 hours,and decreased to a low level at 24 hours,and the number of Bcl-2 positive cells in NAS group was significantly higher than that in RIRI group at each time point,the differences were statistically significant (all P ＜ 0.05).There were almost no Bax positive cells in the retina of the control normal group,and the Bax positive cells were found to be higher of the RIRI group at the 6 hours after RIRI,and reached the higher level at 24 hours,and decreased at 48 hours.The Bax positive cells of NAS group were significantly less than those in the RIRI group at different time points,and the differences were statistically significant (all P ＜0.05).Conclusion NAS can promote the expression of Bcl-2 protein in rat retina after RIRI,inhibit the expression of Bax protein,decrease the expression of active caspase-3 protein,alleviate cell apoptosis,and have neuroprotective effects.关键词
N-乙酰-5-羟色胺/视网膜缺血再灌注损伤/活性Caspase-3/Bcl-2/BaxKey words
N-acetylserotonin/retinal ischemia-reperfusion injury/active caspase-3/Bcl-2/Bax分类
医药卫生引用本文复制引用
张婷婷,赵岩松,王海宇,杨明,程丹丹,牟青杰,王晓莉..N-乙酰-5-羟色胺(NAS)对视网膜缺血再灌注损伤(RIRI)大鼠视网膜活性Caspase-3、Bcl-2、Bax表达的影响[J].眼科新进展,2017,37(8):701-704,708,5.基金项目
国家自然科学基金资助(编号:81000268) （编号:81000268）
山东省自然科学基金项目(编号:ZR2013HL067、ZR2014HQ077、ZR2014-JL049) （编号:ZR2013HL067、ZR2014HQ077、ZR2014-JL049）
山东省医药卫生科技发展计划项目(编号:2013WS0295) （编号:2013WS0295）
山东省高等学校科技计划项目(编号:J15LL08)National Natural Science Foundation of China (No:81000268) （编号:J15LL08）
Natural Science Foundation of Shandong Province (No:ZR2013HL067,ZR2014HQ077,ZR2014JI049) （No:ZR2013HL067,ZR2014HQ077,ZR2014JI049）
Medical and Health Technology Development Program of Shandong Province (No:2013WS0295) （No:2013WS0295）
Shandong Province Science and Technology Project of Higher Education (No:J15LL08) （No:J15LL08）
