眼科新进展2017，Vol.37Issue(8)：705-708,4.DOI:10.13389/j.cnki.rao.2017.0179
不同浓度肽聚糖对角膜上皮细胞Toll样受体(TLR)2和TLR4表达的影响
Effects of peptidoglycan with different concentrations on TLR2 and TLR4 in corneal epithelial cells of mice
摘要
Abstract
Objective To investigate the effects of peptidoglycan (PGN) with different concentrations on Toll-like receptor 2 (TLR2),Toll-like receptor 4 (TLR4) expression in corneal epithelial cells of mice.Methods Corneal epithelial cells of c57 mice were cultured in vitro.Cells were divided into blank control group and 10 mg · L-1 group,30 mg · L-1 gruop and 80 mg · L-1 group (treated by different concentration of PGN for 12 hours).In the meantime,the cells in 30 mg · L-1 group were cultured for different times(named 12 hours group,24 hours group,36 hours group).Expressions of TLR2 and TLR4 mRNA and protein in different group were measured by RT-PCR and flow cytometry.Results Compared with control group (1.00 ± 0.14,1.00 ± 0.01),the expression of TLR2,LR4 mRNA in 10 mg · L-1 group (4.35 ± 0.46,3.53 ± 0.50),30 mg · L-1 group (8.06 ±0.72,5.31 ±0.34),80 mg · L-1 group (2.93 ±0.46,2.23 ±0.04) were increased,the differences were statistically significant (all P ＜ 0.05).Compared with control group,the expression of TLR2,TLR4 protein in different concentration group and 12 hour group were increased,the differences were statistically significant (all P ＜ 0.05).Conclusion PGN can up-regulate both mRNA and protein expression of TLR2 and TLR4 in corneal epithelial cells of mice,suggest that TLR2 and TLR4 in the corneal epithelial cell can recognize some exogenous pathogen and regulate the inflammatory reaction,which are closely related to the occurrence and development of infectious keratitis.关键词
肽聚糖/Toll样受体家族/角膜上皮细胞/感染性角膜炎Key words
peptidoglycan/Toll like receptor 2/Toll like receptor 4/infectious keratitis分类
医药卫生引用本文复制引用
刘晶,白浪,苏亚茹,于健,孟婷,陈敏婷..不同浓度肽聚糖对角膜上皮细胞Toll样受体(TLR)2和TLR4表达的影响[J].眼科新进展,2017,37(8):705-708,4.基金项目
国家自然科学基金资助(编号:81170887) （编号:81170887）
南方医科大学南方医院横向课题基金资助(编号:G201202)National Natural Science Foundation of China (No:81170887) （编号:G201202）
Horizontal Topic Matching Funds of Nanfang Hospital of Southern Medical University(No:G201202) （No:G201202）
