羊膜泪道支架预防去势雄兔干眼症的实验研究
Experimental study of amniotic lacrimal duct stent used to prevent dry eye of castrated rabbits
摘要
Abstract
Objective To explore the effects of amniotic lacrimal duct stenting on the prevention of dry eye in castrated rabbits.Methods Thirtysix healthy male rabbits were selected,the third eyelid were cut off and antiinfection treatment were given,which were randomly divided into 3 groups (12 cases in each group),the castrated male rabbits models were made.Among them,group A was negative control group,group B was dry eye model group,group C was group of lacrimal amniotic membrane group.At 2 weeks before implantation of amniotic lacrimal duct stent,2 weeks,4 weeks and 6 weeks after implantation,the fluorescent (FL) examination,Western blot,Schirmer I examination,immunofluorescence staining and corneal confocal microscopy were performed.Results The levels of tear secretion and FL in the three groups among different time points were significantly different (F=7.126,P =0.009;F =9.658,P =0.016),and there were significant differences among three groups (F =12.582,P =0.005;F =13.187,P =0.013).The tendency of tear secretion and FL in the three groups were also significantly changed (F =8.531,P =0.007;F =10.652,P =0.019).The epithelial basal cells at 6 weeks after implantation in three groups were 3811 ±414,3820 ± 314,2789 ± 353,and the density of inflammatory cells was 266 ±28,266 ± 29,67 ± 13,there were significant differences among three groups (F =13.442,P =0.012;F =9.231,P =0.021).The K1 6 staining in the duct epithelium were negative,and the expression of α-SMA in the lacrimal duct tissue of group A,B and C was not changed at all time points after implantation of amniotic lacrimal stent,and there was no significant difference (F =14.681,P =0.002).Conclusion The amniotic lacrimal stent implantation has certain effect on the prevention of dry eye in rabbit.关键词
羊膜泪道支架/干眼症/去势雄兔Key words
amniotic lacrimal duct stent/xerophthalmia/castrated rabbits分类
医药卫生引用本文复制引用
马明洋,邵毅,袁晴,刘启,刘康成,朱佩文,康红花,姜楠,叶蕾,裴重刚..羊膜泪道支架预防去势雄兔干眼症的实验研究[J].眼科新进展,2017,37(8):709-713,5.基金项目
国家自然科学基金资助(编号:81660158、81160118、81160148、81460092、81400372、81400424) （编号:81660158、81160118、81160148、81460092、81400372、81400424）
江西省远航工程(编号:2014022) （编号:2014022）
江西省自然科学基金重大项目(编号:2016ACB21017) （编号:2016ACB21017）
江西省青年科学基金(编号:20151BAB215016、20161BAB215198) （编号:20151BAB215016、20161BAB215198）
江西省重点研发项目(编号:20151BBG70223) （编号:20151BBG70223）
江西省教育厅重点项目(编号:GJJ160020) （编号:GJJ160020）
江西省教育厅科技计划项目(编号:GJJ160122) （编号:GJJ160122）
江西省卫计委科技计划项目(编号:20164017、20175115、20175116):江西省卫计委中医药科技计划项目(编号:20150823)National Natural Science Foundation of China (No:81660158,81160118,81160148,81460092,81400372,81400424) （编号:20164017、20175115、20175116）
Jiangxi Province Voyage Project(No:2014022) （No:2014022）
Natural Science Key Project of Jiangxi Province (No:20161ACB21017) （No:20161ACB21017）
Youth Science Foundation of Jiangxi Province(No:20151BAB215016,20161BAB215198) （No:20151BAB215016,20161BAB215198）
Key Research Development Foundation of Jiangxi Province (No:20151 BBG70223) （No:20151 BBG70223）
Key Education Department Foundation of Jiangxi Province(No:GJJ160020) （No:GJJ160020）
Education Department Foundation of Jiangxi Province (No:GJJ160122) （No:GJJ160122）
Health Development Planning Commission Science Foundation of Jiangxi Province (No:20164017,20175115,20175116) （No:20164017,20175115,20175116）
Health Development Planning Commission TCM Science Foundation of Jiangxi Province(No:20150823) （No:20150823）
