眼科新进展2017，Vol.37Issue(8)：714-718,5.DOI:10.13389/j.cnki.rao.2017.0181
间充质干细胞对兔自身免疫性干眼模型病理改变及细胞因子表达的影响
Effects of mesenchymal stem cells on pathological changes and cytokines expression in rabbit model of autoimmune dry eye
摘要
Abstract
Objective To establish the model of autoimmune dry eye and analyze the level of cytokines and the pathological changes of autoimmune dry eye rabbits treated with human umbilical cord mesenchymal stem cells (MSCs).Methods Twentyfour female New Zealand rabbits were randomly divided into three groups:Normal control group,dry eye group and MSCs treatment group,8 cases in each group.After treated with MSCs for six weeks,the pathological changes were observed,and the expression of Th1 and Th17 cell differentiation related cytokines mRNA in lacrimal gland tissue was detected by real-time quantitative PCR.The percentage of regulatory T cells (CD4 +Foxp3 + cells) in the lacrimal gland and spleen tissues of the dry eye model group and MSCs treatment group was measured by flow cytometry.Results After treatment with MSCs for 6 weeks,HE staining from each rabbit lacrimal gland showed that,the normal control group showed no or only a few lymphocytes;The lacrimal gland cells were atrophied in the dry eye group,the distribution of lymphocyte scattered around the gland and small blood vessels;Compared with dry eye group,the lacrimal gland lymphocyte infiltration reduced in MSCs treatment group,and the cell morphology were better.Compared with dry eye group,lymphocyte infiltration and aggregation in the conjunctival conjunctiva in MSCs treatment group were significantly reduced,the epithelial structure was intact,and the degeneration and atrophy cells were rare.The expression of inflammatory factors including interferon-γ(IFN-γ),Interleukin-17 (IL-17) and transcription factor T-bet mRNA were decreased in MSCs treatment group,there were significant difference compared with dry eye group (all P ＜ 0.05);Thl7 cell associated cytokines IL-17 expression levels were decreased,but no significant difference was found between two groups (P ＞ 0.05);The expression of transcription factor RORrt mRNA in dry eye group was significantly decreased,the difference was statistically significant (P ＜ 0.05).The expression of inflammatory factors TNF-α in the lacrimal gland tissue of MSCs treatment group was significantly lower than that in the dry eye model group,while the expression of the anti-inflammatory factor TGF-β was significantly higher than that in the dry eye model group,and the differences were statistically significant (all P＜0.05).The regulatory T cells (CD4 + Foxp3 + cells) in the lacrimal gland of dry eye model group accounted for 10％ of lymphocytes was,while the treatment group accounted for 27.8％,MSCs group was significantly higher than dry eye group,the difference was statistically significant (P ＜ 0.05).Conclusion MSCs can reduce the histopathological changes of the immune dry eye,and they may have immunoregulatory effects on autoimmune dry eye.The mechanisms of immunomodulatory effects may be related to the balance of inflammatory cytokines and anti-inflammatory cytokines.关键词
间充质干细胞/兔自身免疫性干眼/组织病理学/细胞因子Key words
mesenchymal stem cells/autoimmune dry eye rabbit/histopathology/cytokines分类
医药卫生引用本文复制引用
王希莲,路晓晓,杨丽媛,粘红,杨丹,魏瑞华..间充质干细胞对兔自身免疫性干眼模型病理改变及细胞因子表达的影响[J].眼科新进展,2017,37(8):714-718,5.基金项目
国家自然科学基金资助(编号:81100646) （编号:81100646）
天津市科委自然科学基金资助(编号:11JCYBJC26000,13JCYBJC23300) （编号:11JCYBJC26000,13JCYBJC23300）
教育部留学回国人员科研启动基金资助项目(编号:第48批) （编号:第48批）
天津市北辰区科技发展计划项目(编号:BC2014-10)National Natural Science Foundation of China (No:81100646) （编号:BC2014-10）
Natural Science Foundation of Tianjin Science Committee(No:11JCYBJC26000,13JCYBJC23300) （No:11JCYBJC26000,13JCYBJC23300）
the Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry(No:48) （No:48）
Science and Technology Foundation of Beichen District of Tianjin (No:BC2014-10) （No:BC2014-10）
