眼科新进展2017，Vol.37Issue(8)：719-722,727,5.DOI:10.13389/j.cnki.rao.2017.0182
转基因荧光小鼠视神经轴索钝性损伤后退行性病变观察
Neurodegenerative disorder after optic nerve crush in transgenic fluorescence mice
摘要
Abstract
Objective To observe optic nerve axons degenerative disorder and microglial responses by establishing unilateral optic nerve crush model.Methods YFP mouse group with axonal markers and GFP mouse group with microglia markers were divided into surgery and control group,the optic nerve were dissected at 4 hours,1 day,3 days,5 days,10 days after optic nerve crush,and the neuronal degenerative disorder and microglial responses were observed by confocal laser scanning microscope.Rrsults Compared with control group,the optic nerve axons in YFP mouse group were fractured in injury region at postoperative 4 hours;The partial axon became beadlike change at postoperative 1 day;Most of the axons turned into the process of beadlike change at postoperative 3 days;The axons became to debris from beadlike at postoperative 5 days;The axons changed into many debris at postoperative 10 days.Compared with control group,the formation of glial scar and resting microglia in GFP mouse group began to emerge at postoperative 4 hours;The microglia gradually activated and began to cover the injury region at postoperative 1 day;The activated miacroglia basically covered the injury region at postoperative 3 days;The number of microglia roughly remained stable,although the axons continued to deteriorate at postoperative 5 days and 10 days.Conclusion The optic nerve occur irreversible degenerative disorder after being injured,meanwhile with the microglial increase and activation.This phenomenon suggests that microglia is closely associated with optic nerve degeneration.关键词
GFP小鼠/YFP小鼠/视神经损伤/小胶质细胞Key words
GFP mouse/YFP mouse/optic nerve lesion/microglia分类
医药卫生引用本文复制引用
孔涛,王兰兰,高岚..转基因荧光小鼠视神经轴索钝性损伤后退行性病变观察[J].眼科新进展,2017,37(8):719-722,727,5.基金项目
国家自然科学基金资助(编号:31471953) National Natural Science Foundation of China (No:31471953) （编号:31471953）
