眼科新进展2017，Vol.37Issue(8)：723-727,5.DOI:10.13389/j.cnki.rao.2017.0183
阈值下577nm微脉冲激光光凝对早期糖尿病大鼠视网膜血管内皮生长因子、神经生长因子和趋化素表达的影响
Effects of subthreshold micropulse yellow laser (577 nm) on VEGF, NGF and chemerin expressions in retina of early stage diabetic rats
摘要
Abstract
Objective To investigate the effects of subthreshold micropulse yellow laser (577 nm) on vascular endothelial growth factor (VEGF),nerve growth factor (NGF) and Chemerin expressions in retina of early stage diabetic rats.Methods A total of 40 Brown Norway rats were treated with streptozocin (65 mg · kg-1) to establish the diabetic model.20 diabetic BN rats' right eyes were received subthreshold micropulse yellow laser (577 run) therapy after 2 weeks.The left eyes were used as control group.At 3 days,7 days,14 days,28 days after laser therapy,5 BN rats were randomly chosen to perform RT-PCR and Weston-blot.The expressions of mRNA and protein of VEGF,NGF and Chemerin were analyzed.Results The expression of VEGF mRNA and protein increased in control group at 3 days,7 days,14 days and 28 days (all P ＜ 0.05).Compared with the control group,VEGF mRNA and protein decreased in the subthreshold micropulse yellow laser (577 nm) group (all P ＜ 0.05).The expression of NGF mRNA and protein decreased in the control group at 3 days,7 days,14 days and 28 days (all P ＜ 0.05),however,the difference was not statistically significant between 3 days and 7 days(P ＞0.05).Compared with control group,NGF mRNA and protein increased in the subthreshold micropulse yellow laser (577 nm) group (all P ＜ 0.05),with maximum expression at 14 days.The expression of chemerin mRNA and protein increased at 3 days,7 days,14 days and 28 days in the control group (all P ＜0.05).Compared with the control group,chemerin mRNA and protein decreased in the subthreshold micropulse yellow laser (577 urn) group (all P ＜ 0.05).Conclusion Subthreshold micropulse yellow laser (577 urn) can suppress VEGF,Chemerin expression and upregulate NGF expression in early stage diabetic rats.关键词
577 nm激光/微脉冲激光/糖尿病/血管内皮生长因子/神经生长因子/趋化素Key words
577 nm laser/micropulse laser/diabetes/vascular endothelial growth factor/nerve growth factor/chemerin分类
医药卫生引用本文复制引用
连海燕,宋艳萍,易敬林,杨海军,罗云峰,杜红岩..阈值下577nm微脉冲激光光凝对早期糖尿病大鼠视网膜血管内皮生长因子、神经生长因子和趋化素表达的影响[J].眼科新进展,2017,37(8):723-727,5.基金项目
国家自然科学基金资助(编号:61378084)National Natural Science Foundation of China (No:61378084) （编号:61378084）
