中国临床药理学与治疗学2017，Vol.22Issue(7)：721-730,10.
基于代谢组学技术对雷公藤多苷和奥硝唑诱导大鼠弱精子症的病理机制研究
Metabolomic study on the mechanism of asthenospermia induced by tripterygium glycosides and ornidazole in rats via GC-MS approach
摘要
Abstract
AIM:To explore the protential biomarkers and the mechanism of asthenospermia induced by tripterygium glycosides (TG) and ornidazole (ONZ),respectively,relying on gas chromatography-mass spectrometry (GC-MS)-based metabolomics.METHODS:Wistar rats were orally administrated with TG(40 mg · kg-1 · d-1) and ONZ (400 mg · kg-1 · d-1) for 30 days,respectively.Serum and epididymal fluid samples were collected every two weeks.A metabolomic approach based on GC-MS was employed to identify endogenous metabolites in serum and epididymal fluid samples,respectively.Data acquired were further evaluated by multivariate statistical analysis and a partial least squares diseriminant analysis (PLS-DA) was applied to finding potential biomarkers.RESULTS:PLS-DA clearly profiled a time-dependent alternation of metabolic phenotype of serum and epididymal fluid samples.The clear classifications between the normal and TG group,the normal and ONZ group as well as TG group and ONZ group were achieved.And some significant metabolites were successfully filtered.The levels of lysine,threonine and some other amino acids in serum were higher in the TGtreated rats than those in the normal rats.In the ONZ-treated rats,concentrations of amino acids in serum were declined.In addition,the level of butanedioic acid was increased in epididymal fluid of TG-treated rats,while the levels of urea,cholesterol,inositol,etc were varied in an opposite manner.Significant changes were observed in the levels of dihydroxyacetone phosphate and ribulose-5-phosphate in epididymal fluid of ONZ-treated rats,compared with the normal rats.CONCLUSION:The metabolomics technology as a high-efficiency tool can be used to distinguish the difference between TG-induced and ONZ-induced asthenospermia in rats.More importantly,TG-induced and ONZ-induced asthenospermia may have different etiology due to disturbing different endogenous metabolic pathways.关键词
代谢组学/弱精子症/雷公藤多苷/奥硝唑/气相色谱-质谱Key words
metabonomics/asthenospermia/tripterygium glycosides/ornidazole/GC-MS分类
医药卫生引用本文复制引用
顾惠琴,马博,赵昂,周艳芬,李静,范丽,王聪,郭素菡,王宇,张琪..基于代谢组学技术对雷公藤多苷和奥硝唑诱导大鼠弱精子症的病理机制研究[J].中国临床药理学与治疗学,2017,22(7):721-730,10.基金项目
国家自然科学基金项目(81373478) （81373478）
