中国临床药理学与治疗学2017，Vol.22Issue(7)：743-748,6.
D-4F在七氟烷处理对急性高糖血症小鼠血管保护中的作用及机制
D-4F enhances sevoflurane-induced vascular protection during acute hyperglycemia
摘要
Abstract
AIM:To investigate the effects of sevoflurane-induced vascular protection by apolipoprotein A-1 mimetic D-4F during acute hyperglycemia.METHODS:Thrty-two male C57BL/6J mice were randomly assigned to 4 groups (n =8).Control group(CON group),D-4Fgroup,acute hyperglycemia group (AHG group),dextrose 2 g/kg (intraperitoneal injection);AHG + D-4F group.Blood glucose,isometric aortic tension,nitric oxide (NO) were measured in mice in the absence or presence of sevoflurane (1.71％).Expressions of eNOS,p-eNOS,Cav-1 in arterioles were determined by Western blot.Superoxide generation species (ROS) were assessed in human umbilical vein endothelial cells (HUVEC) cultured in 5.5 or 20.0 mmol/L glucose with isoflurane (0.5 mmol/L) in the presence or absence of brefeldin A (BFA,4 μg/mL) or D-4F (0.5 μg/mL).RESULTS:Vasodilation,phosphorylations,NO production of eNOS were decreased while Cav-1 expression was increased during acute hyperglycemia.D-4F increased endothelium-dependent vasodilation (P ＜ 0.01) while decreased Cav-1 expression and ROS production in HG + D-4F group after sevoflurane-induction.CONCLUSION:Polipoprotein A-1 mimetic D-4F can enhance sevoflurane-induced vascular protection,decrease Cav-1 expression and ROS production while increase NO production during acute hyperglycemia.关键词
七氟烷/心血管保护/D-4F/急性高糖血症/一氧化氮/活性氧Key words
sevoflurane/vascular protection/D-4F/acute hyperglycemia/NO/ROS分类
医药卫生引用本文复制引用
刘若海,卢园园,李丽伶,沈露露,曹红,李军,李旭..D-4F在七氟烷处理对急性高糖血症小鼠血管保护中的作用及机制[J].中国临床药理学与治疗学,2017,22(7):743-748,6.基金项目
浙江省自然科学基金青年项目(LQ17H020004) （LQ17H020004）
浙江省教育厅一般科研项目(Y201431185) （Y201431185）
温州市公益性科技计划项目(Y20160368) （Y20160368）
