中国临床药理学与治疗学2017，Vol.22Issue(7)：755-761,7.
当归挥发油联合卡铂对人肺腺癌A549细胞增殖及凋亡的影响
Effect of volatile oil of the angelica combined with carboplatin on the proliferation and apoptosis of human lung adenocarcinoma cell A549
摘要
Abstract
AIM:To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell A549 combined with carboplatin.METHODS:Use the MTT colorimetric method to investigate cytotoxicity effect of volatile oil of the angelica alone or combined with carboplatin to human lung adenocarcionma cell A549;Use flow cytometry (FCM)analysis the cell apoptosis.Observe the cell apoptosis by AO/BE fluorescent staining method.RESULTS:The A549 cell was inhibited by volatile oil of the angelica alone or combined with carboplatin in a concentration-dependent manner,and compared with use volatile oil of the angelica alone,the IC50 descend in drug combination group.Results of flow cytometry indicates that,volatile oil of the angelica alone or combined with carboplatin,could induce apoptosis and necrosis of A549 cell,and compared with use volatile oil of the angelica alone,the rate of apoptosis and necrosis all rise in drug combination group.The results of AO/BE fluorescent staining method displayed that,the rate of apoptosis and necrosis were all raised in drug combination group.CONCLUSION:Volatile oil of the angelica had depressant effect on the generation of A549 cell,there is a synergistic cytotoxic effect on A549 cells treated with volatile oil of the angelica combined with carboplatin.The mechanism is probably that it can induce apoptosis of A549 cell.关键词
当归挥发油/卡铂/人肺腺癌A549细胞/细胞凋亡Key words
volatile oil of the angelica/carboplatin/human lung adenocarcinoma cell A549/apoptosis分类
医药卫生引用本文复制引用
张艳霞,张正顺,陈绍仪,孔学礼,周佳玮..当归挥发油联合卡铂对人肺腺癌A549细胞增殖及凋亡的影响[J].中国临床药理学与治疗学,2017,22(7):755-761,7.基金项目
白银市科技计划项目(2013-2-76Y) （2013-2-76Y）
甘肃中医学院中青年科研基金项目(2305016601) （2305016601）
