中国临床药理学与治疗学2017，Vol.22Issue(7)：775-778,4.
依托咪酯对大鼠海马兴奋性突触传递的影响
Effect of etomidate on excitatory synaptic transmission efficacy in rat hippocampus
摘要
Abstract
AIM:To investigate the effect of etomidate on excitatory synaptic transmission efficacy in stratum radiatum of CA1 area of rat hippocampus and the underlying mechanism.METHODS:Twenty-four adult male Wistar rats were randomly divided into 4 groups (n =6 each):control group,bicuculline group,etomidate group and etomidate + bicuculline group.The animals were anesthetized with intraperitoneal (IP) pentobarbital 50 mg/kg.The stimulating and recording electrodes were inserted into the stratum radiatum of CA1 area of the hippocampus.The paired pulse stimulation with the interval 30 ms between the 2 pulse stimulation was used.Excitatory post-synaptic potential (EPSP) was elicited in vivo before (baseline) and after IP bicuculline (2 mg/kg) or/and etomidate (10 mg/kg)administration.The EPSP2/EPSP1 (E2/E1) ratio was recorded during paired pulse stimulation.RESULTS:Compared with control group,the E1 amplitude was significantly decreased in etomidate group (P ＜ 0.05),but no significant change was found in bicuculline group and etomidate + bicuculline group (P ＞ 0.05).Compared with control group,E2 and E2/E1 ratio were significantly increased in bicuculline group,while significantly decreased in etomidate group (P ＜ 0.05).E2 and E2/E1 ratio were significantly lower in etomidate group and etomidate + bicuculline group than in bicuculline group (P ＜ 0.05).CONCLUSION:The results suggest that etomidate might depress the excitatory synaptic transmission efficacy in the CA1 area of the rat hippocampus through presynaptic GABA energic inhibitory circuit.关键词
依托咪酯/突触传递/海马Key words
etomidate/synaptic transmission/hippocampus分类
医药卫生引用本文复制引用
魏辉明,刘超,杨云丽..依托咪酯对大鼠海马兴奋性突触传递的影响[J].中国临床药理学与治疗学,2017,22(7):775-778,4.基金项目
成都军区“十二五”医学科研项目(B14013) （B14013）
