中国输血杂志2017,Vol.30Issue(6):586-589,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.06.009
Mur血型抗原抗体调查及应用研究
The investigation and application of Mur blood group antigen and antibody
摘要
Abstract
Objective To investigate the frequency of anti-Mur antibodies in the population of Guangdong province,analyze the properties and sources of these antibodies and establish Mur blood bank for clinical use.Methods Anti-Mur antibodies were screened by the saline agglutination test and the polybrene method in microplate.Mur antigen RBCs phenotypes were detected with anti-Mur antibodies by the saline agglutination test and the polybrene method in microplate.Part of samples were detected by solid phase immune adsorption test (SPIA).Results 34 419 samples of blood donors from 5 cities (Zhongshan,Shenzhen,Jiangmen,Dongguan,Huizhou) in Guangdong province were detected and 48 cases were anti-Mur positive (0.14%,48/34 419).The proportion of IgM was 79.2% (38/48),IgM+IgG was 16.7% (8/48),IgG was 4.2% (2/48).There was no significant difference in anti-Mur frequency of blood donors among the 5 cities,so did anti-Mur frequency between the patients and blood donors.The total positive rate of Mur antigen was 7.24% (208/2 872),including 7.31% in blood donors (128/1 752) and 7.14% in patients (80/1 120) (P>0.05).The Mur positive people i were all Han people,66.7% of examinee's native place were in Guangdong.We established Mur blood bank.Four cases of anti-Mur were detected from 2 541 blood samples with SPIA method.Conclusion Anti-Mur antibodies has clinical significance at 37 degrees,and they could be detected with SPIA method.The frequencies of anti-Mur antibodies and Mur antigens were significantly higher than that of anti-D antibody incidence and RhD antigen negative blood types in the population of Guangdong area Mur blood group should be examined and Mur positive panel red blood cells should be used in irregular antibody detection,in order to improve the safety of transfusion.关键词
Mur血型/抗-Mur/不规则抗体筛查/固相免疫吸附试验/Mur稀有血型库Key words
Mur blood group/anti-Mur/irregular antibody screening/solid phase immunosorbent assay/Mur blood bank分类
医药卫生引用本文复制引用
孙爱农,李勇,段生宝,易峰,廖艳婷,丁少华,曾劲峰,何子毅,古锦萍,钟展华..Mur血型抗原抗体调查及应用研究[J].中国输血杂志,2017,30(6):586-589,4.基金项目
中山市科技计划项目(2014A1FC045),江苏省自然科学基金(BK20130343) (2014A1FC045)