中国输血杂志2017，Vol.30Issue(6)：600-603,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.06.013
广州地区伊朗献血人群CD36表型筛查及基因型鉴定
The CD36 expression screening and genotype detection in Iranian blood donors in Guangzhou , China
摘要
Abstract
Objective To learn about the phenotype and genotype of CD36 in blood donors among Iranians in Guangzhou district.Methods 53 Iranian blood donors in Guangzhou were enrolled in 2016.The CD36 expression both on platelets and monocytes was detected by flow cytometry.The DNA was extracted from the samples with abnormal CD36 expression,and its genotype was detected by PCR-SBT,the gene mutation was analyzed further.Results The frequency of abnormal CD36 expression In the 53 Iranian blood donors is 7.55％ (4/53),including 2 with low CD36 expression on platelets,1 with CD36 deficiency expression both on platelets and monocytes (type Ⅰ deficiency),1 with low CD36 expression on monocytes.So the frequency of CD36 deficiency was 1.89％ (1/53).In the results of PCR-SBT for the 4 samples,2 samples were CD36 gene mutations,exon 10-975T/G and exon1 1 del CTA respectively(new mutations both),the other 2 ones were normal CD36 genotypes.Conclusions The new 2 gene mutations have been found,and their efficiencies on expression of CD36 antigen need to be confirmed further.关键词
CD36表型/CD36基因/CD36抗原缺失/血小板糖蛋白Ⅳ/基因突变/伊朗人Key words
platelet membrane glycoprotein Ⅳ/CD 36 gene/CD36 deficiency/gene mutation/Iranians/blood donor分类
医药卫生引用本文复制引用
王嘉励,叶欣,刘静,丁浩强,徐秀章,邓晶,邵媛,陈扬凯,夏文杰,陈大伟..广州地区伊朗献血人群CD36表型筛查及基因型鉴定[J].中国输血杂志,2017,30(6):600-603,4.基金项目
广东省医学科研基金(2015316),广州市血液安全重点实验室项目(201509010009),广州市科技创新委员会项目(201607010007),广东省自然科学基金(2016A030313123,2016A030313124). （2015316）
