中国输血杂志2017，Vol.30Issue(6)：604-607,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.06.014
基于转录介导扩增的单人份核酸检测在血液筛查中的应用分析
Application and analysis of transcription-mediated amplification based individualdonation nucleic acid testing for blood screening in Chongqing
摘要
Abstract
Objective This study aims todiscuss the application of transcription-mediated amplification (TMA) based individualdonation nucleic acid testing (ID-NAT)for blood screening in Chongqing and analyze its role and significance in improving thesafety of clinical transfusion.Methods Specimen were collected from 128 973 volunteer blood donors datingfrom January 1st,2015 to December 31th,2015.Enzyme-linked immunosorbent assay (ELISA) were performed twice(domestic reagentsbeing the 3rd gen and imported reagent being the 4th) to identify HBsAg,anti-HCV,anti-HIV-1/2 and HIV-1p24 antigenpresence.Meanwhile,TMA basedID-NAT was conducted for HBV/ HIV-1/HCV detection.ELISA non-reactive but NAT reactive samples were then discriminated with necessary reagents.Results 885 out of 128 973 samples were confirmed NAT reactive,yielding are active rate of 0.69％ (885/128 973).528 of which presented dual-reactivity to ELISA and NAT while the rest 357 samples were ELISA non-reactive but NAT reactive with a NAT-only reactive rate of 0.28％ (357/ 128973).These 357 samples were further tested with discrimination assays,which confirmed 114 HBVDNA reactive,3 HIV-1 RNA reactive and 0 HCV RNA reactive samples with a discrimination reactive rate of 32.77％ (117/357).Three HIV-1 RNA reactive blood donors were followed up,and eventually confirmed as "window period" infection.Conclusion 114 HBV DNA reactive samples and 3 HIV "window period" blood infections were successfully detected and screened by NAT,which fully demonstrated the necessity of virus nucleic acid detection in blood screening and its important roleinblood safety.关键词
核酸检测/转录介导的扩增/血液筛查/献血者/检测窗口期Key words
transcription-mediated amplification (TMA)/nucleic acid testing (NAT)/blood screening/window period分类
临床医学引用本文复制引用
尹丹,毕蕾静,黄秀琳,刘东,田耘博,王芳,李维..基于转录介导扩增的单人份核酸检测在血液筛查中的应用分析[J].中国输血杂志,2017,30(6):604-607,4.基金项目
重庆市卫生和计划生育委员会医学高端后备人才培养项目(2015HBRC013) （2015HBRC013）
