中国输血杂志2017，Vol.30Issue(6)：611-614,4.DOI:10.13303/j.cjbt.issn.1004-549x.2017.06.016
献血者HIV RNA窗口期标本的追踪和随访
Tracking and follow-up study of an HIV RNA window period blood donor
李维 1尹丹 1毕蕾静 1刘东 1韩治国 1张蕾 1秦伟斐 1欧阳熊妍1
作者信息
摘要
Abstract
Objective To track,follow-up and verify a HIV RNA window period blood donor.Methods The donor blood sampleswere collected at different stagesduringthe window period (every 4-8days depending on thedonor status).Tracking,detection and follow-up study were performed usingthe 3rd and the 4th generation enzyme Linked immunosorbentassay(ELISA) reagents,qualitative and quantitative nucleic acid tests(NAT).Routine serological testswith two ELISA reagents was performed while NATsingle assay were conducted forHBV,HCV and HIVtests.Results The first ELISAtestresultwasnon-reactivewhilethe qualitative NAT was reactive.The quantitative NAT and West Blot (WB) results were,however,both non-reacitve.We tracked andtested thedonor's blood samples 4 timestill the serologicalresults turnedreactive after three weeks.Thus acquiringa complete processofserological conversion.Conclusion It was the first time to track、follow-up,test and confirm ahighvirusload donorin an HIV RNA window period in Chongqing area.NAT technology is capable of blockingsuchinfectedblood sourcefrom clinicaluse.关键词
HIV窗口期/献血者追踪检测/献血者随访/核酸检测/ELISA/WB确认试验/血液筛查Key words
HIV Window Period/DonorTracking and Detection/DonorFollow-up study/Nucleic Acid Testing (NAT)/Enzyme Linked ImmunoSorbentAssay (ELISA)/West Blot (WB)/Blood Screening分类
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李维,尹丹,毕蕾静,刘东,韩治国,张蕾,秦伟斐,欧阳熊妍..献血者HIV RNA窗口期标本的追踪和随访[J].中国输血杂志,2017,30(6):611-614,4.
