中国比较医学杂志2017，Vol.27Issue(8)：16-21,6.DOI:10.3969.j.issn.1671-7856.2017.08.004
异常黑胆质证2型糖尿病大鼠模型的血浆代谢组学研究
Plasma metabonomic study of abnormal Savda rat model of type 2 diabetes
摘要
Abstract
Objective To study the serum metabonomic changes in abnormal Savda rat model of type 2 diabetes mellitus (T2DM).Methods Sprague Dawley (SD) rats were subjected to abnormal Savda-producing conditions followed by induction of T2DM.Plasma samples were collected from each test group and analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy.The spectral profiles were analyzed by multivariate analysis using orthogonal projection to latent structurs discriminant analysis (OPLS-DA).Results The content of glucose and lactic acid in plasma of the rats with abnormal Savda syndrome was decreased, while the contents of VLDL, LDL and carnitine increased obviously.For abnormal Savda rat model with T2DM, the concentration of amino acids (alanine, valine, isoleucine, leucine, tyrosine, glutamic acid, phenylalanine, methyl-histidine, glycine), and lactic acid, pyruvic acid, glycoprotein, citric acid, creatinine, β-hydroxybutyric acid, acetoacetic acid, acetic acid, acetate, creatine, carnitine, scyllo-inositol, VLDL, LDL were decreased, and glucose and taurine concentrations were increased significantly.Conclusions Protein metabolism, phenylalanine and tyrosine metabolic pathway, glycolytic pathway and the lipid metabolic pathway may be considered as biomarkers of abnormal Savda rat model of T2DM.关键词
异常黑胆质证/2型糖尿病/动物模型/代谢组学Key words
Abnormal Savda/Type 2 diabetes/Animal model/Metabolomics分类
医药卫生引用本文复制引用
巴哈古力·阿卜都热合曼,阿布力克木·毛拉尤甫,买吾拉尼江·依孜布拉,马红,马晓丽,巴吐尔·买买提明..异常黑胆质证2型糖尿病大鼠模型的血浆代谢组学研究[J].中国比较医学杂志,2017,27(8):16-21,6.基金项目
国家自然科学基金(81460749). （81460749）
